Purified anti-mouse CD43 Antibody

Pricing & Availability
Clone
S11 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48
Isotype
Rat IgG2b, κ
S11_Pure_021612
C57BL/6 mouse splenocytes were stained with either purified anti-mouse CD43 (clone S11) (filled histogram) or rat IgG2b isotype control (dashed histogram), followed by a anti-rat IgG FITC.
  • S11_Pure_021612
    C57BL/6 mouse splenocytes were stained with either purified anti-mouse CD43 (clone S11) (filled histogram) or rat IgG2b isotype control (dashed histogram), followed by a anti-rat IgG FITC.
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143201 50 µg $65.00
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143202 500 µg $276.00
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Description

CD43, also known as Leukosialin and Ly48, is a 125 kD sialoprotein (glycosylated protein) expressed from 1.2 kBase mRNA in bone marrow-derived cells. This occurs early in development. Cells expressing CD43 include γ/δ T cells, macrophages, mature B cells, and dendritic cells. CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia. The intracellular distribution of CD43 is determined by binding to moesin, an intracellular membrane protein, which is in-turn bound in some manner to the actin cytoskeleton. Defects with CD43 function and expression retard cellular locomotion, resulting in a wide range of immune disorders. Wiscott-Alderich syndrome, and the varying degrees of its severity, is related to the dysregulation of CD43 expression.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting3. The S11 antibody reacts with pan-CD43.

Application References

(PubMed link indicates BioLegend citation)
  1. Gaspari AA, et al. 1993. J. Invest. Dermatol. 100:247. (FC)
  2. Merzaban JS, et al. 2005. J. Immunol. 174:4051. (FC)
  3. Baecher-Allan CM, et al. 1993. Immunogenetics. 37:183. (WB)
Product Citations
  1. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  2. Blriot C, et al. 2021. Immunity. :. PubMed
  3. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  4. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
RRID
AB_11124103 (BioLegend Cat. No. 143201)
AB_11124103 (BioLegend Cat. No. 143202)

Antigen Details

Structure
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites; 115 kD and 130 kD glycoforms with different glycosylation and sialylation.
Distribution

Gamma/delta T cells, macrophages, mature B cells, and dendritic cells.

Function
Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
Interaction
CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia.
Ligand/Receptor
Moesin, CD54, E-selectin, Siglec-1.
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
6. Matsumoto M, et al. 2005. J. Immunol. 175:8042.

Gene ID
20737 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org
Go To Top Version: 2    Revision Date: 06/09/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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