- Clone
- QA19A52 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD45R
- Isotype
- Rat IgG2a, κ
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 FITC and purified anti-mouse CD45R/B220 recombinant (clone QA19A52) (left) or rat IgG2a, κ isotype (right) followed by APC anti-rat IgG. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse CD45R/B220 recombinant (clone QA19A52) and Alexa Fluor® 594 anti-mouse CD3ε (clone 500A2) (green) overnight at 4°C. On the next day, sections were washed and stained with 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 111302 | 100 µg | $88.00 | |||
CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker. CD19, however, may be more appropriate for B cell specificity.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2927819 (BioLegend Cat. No. 111302)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
B cells, T cell subset, NK cell subset
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Trowbridge IS, et al. 1994. Annu Rev Immunol. 12:85-116.
- Kishihara K, et al. 1993. Cell 74:143-56.
- Pulido R, et al. 1988. J Immunol. 140:3851-7.
- Gene ID
- 19264 View all products for this Gene ID 5788 View all products for this Gene ID
- UniProt
- View information about CD45R on UniProt.org
Other Formats
View All CD45R/B220 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD45R/B220 Recombinant Antibody | QA19A52 | FC,IHC-F |
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Purified anti-mouse CD45R/B220 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... 
C57BL/6 mouse frozen spleen section was fixed with 4% parafo...