Purified anti-mouse CD45RB Antibody

Pricing & Availability
Clone
C363-16A (See other available formats)
Regulatory Status
RUO
Other Names
CD45RB
Isotype
Rat IgG2a, κ
C363-16A_Pure_091907
C57BL/6 mouse splenocytes stained with purified C363-16A, followed by anti-rat IgG FITC
  • C363-16A_Pure_091907
    C57BL/6 mouse splenocytes stained with purified C363-16A, followed by anti-rat IgG FITC
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Cat # Size Price Quantity Check Availability
103302 500 µg $229.00
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Description

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cloned mouse Th2 cell lines
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Lopes N, et al. 2021. Nat Immunol. 22:179. PubMed
  2. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
RRID
AB_313009 (BioLegend Cat. No. 103302)

Antigen Details

Structure
Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution

Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells

Function
Phosphatase, T cell development and activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, Thy1
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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