- Clone
- OX-79 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- OX-79
- Isotype
- Rat IgM, κ
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C57BL/6 splenocytes stained with purified OX-79 conjugated to FITC and CD4 (GK1.5) PE -
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 124701 | 50 µg | $71.00 | |||
CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BAB/14 mouse macrophage cell line RAW264
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
- Puls KL, et al. 2002. Int. Immunol. 14(3):249.
- RRID
-
AB_1937288 (BioLegend Cat. No. 124701)
Antigen Details
- Structure
- A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
- Distribution
-
Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
- Function
- Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
- Interaction
- Interact with CD2, CD9, CD19, and CD21.
- Cell Type
- B cells, Leukocytes, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Camo AM, et al. 1995. Eur. J. Immunol. 25(7):2090
2. Tomlinson MG, et al. 1995. Eur. J. Immunol. 25(8):2201
3. Angelisova P, et al. 1994. Immunogenetics. 39(4):249
4. Wright MD, et al. 1993. Int. Immunol. 5(2):209
5. Rasmussen A, et al. 1994. J. Immunol. 153:4997 - Gene ID
- 12508 View all products for this Gene ID
- UniProt
- View information about CD53 on UniProt.org
Other Formats
View All CD53 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD53 | OX-79 | FC,WB |
| FITC anti-mouse CD53 | OX-79 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD53
C57BL/6 splenocytes stained with purified OX-79 conjugated t... 
C57BL/6 splenocytes stained with rat IgM FITC isotype contro... -
FITC anti-mouse CD53
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) ...
C57BL/6 splenocytes stained with rat IgM FITC isotype contro...