- Clone
- W21055C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ICAM-1, Ly-47
- Isotype
- Rat IgG2a, κ
-
C57BL/6 mouse splenocytes stained with purified anti-mouse CD54 (clone W21055C) (filled histogram), or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE. -
IHC staining of anti-mouse CD54 (clone W21055C) on C57BL/6 mouse frozen small intestine. After fixing with 4% paraformaldehyde (PFA) for ten minutes, the tissue was incubated with 10 µg/mL of purified anti-mouse CD54 (clone W21055C) overnight at 4°C, followed by incubation with 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) (Cat. No. 405422) for two hours at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. -
IHC staining of anti-mouse CD54 (clone W21055C) on C57BL/6 mouse frozen brain tissue (negative control). After fixing with 4% paraformaldehyde (PFA) for ten minutes, the tissue was incubated with 10 µg/mL of purified anti-mouse CD54 (clone W21055C) overnight at 4°C, followed by incubation with 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) (Cat. No. 405422) for two hours at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 165902 | 100 µg | $105.00 | |||
CD54, also known as ICAM-1 and Ly-47, is a 90 kD immunoglobulin superfamily member. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse ICAM-1 (28-485) with human Fc chimera plus transfectant (full length)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemal staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3097209 (BioLegend Cat. No. 165902)
Antigen Details
- Structure
- Ig superfamily, 90 kD
- Distribution
-
Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
- Function
- Immune reaction, inflammation, adhesion
- Ligand/Receptor
- CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Springer TA. 1990. Nature. 346:425-34.
- Rothlein R, et al. 1986. J Immunol. 137:1270.
- Gene ID
- 15894 View all products for this Gene ID
- UniProt
- View information about CD54 on UniProt.org
Other Formats
View All CD54 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD54 | W21055C | FC,IHC-F |
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