- Clone
- W20055M (See other available formats)
- Regulatory Status
- RUO
- Other Names
- E-selectin, ELAM-1, LECAM-1
- Isotype
- Rat IgG2b, κ
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bEnd.3 cells (mouse endothelial cells) were stained with purified anti-mouse CD62E (E-selection) (clone W20055M) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by anti-rat IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 132402 | 100 µg | $135.00 | |||
CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Transfectant cells (29-564 aa) and recombinant protein (29-564 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Flow cytometry testing showed that clone W20055M does not affect binding of clone W20055P on target cells.
- RRID
-
AB_3097461 (BioLegend Cat. No. 132402)
Antigen Details
- Structure
- Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
- Distribution
-
Highly expressed on activated endothelial cells
- Function
- Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
- Ligand/Receptor
- Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- 20339 View all products for this Gene ID
- UniProt
- View information about CD62E on UniProt.org
Other Formats
View All CD62E Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD62E (E-selectin) | W20055M | FC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD62E (E-selectin)
bEnd.3 cells (mouse endothelial cells) were stained with pur...