Purified anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
S18017D (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Rat IgG2a, κ
S18017D_PURE_CD64_FcgammaRI_Antibody_030821
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and purified anti-mouse CD64 (FcγRI) (clone S18017D) (left) or purified anti-rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG PE. Data shown was gated on myeloid cells.
  • S18017D_PURE_CD64_FcgammaRI_Antibody_030821
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and purified anti-mouse CD64 (FcγRI) (clone S18017D) (left) or purified anti-rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG PE. Data shown was gated on myeloid cells.
  • S18017D_PURE_CD64_FcgammaRI_Antibody_2_102821
  • S18017D_PURE_CD64_FcgammaRI_Antibody_3_102821
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD64 (FcγRI) Antibody (clone S18017D) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
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Cat # Size Price Quantity Check Availability
161002 100 µg $118.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse FcγRI/CD64 (Cat. No. 773806)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yull S, et al. 2023. Front Immunol. 1276979:14. PubMed
RRID
AB_2890725 (BioLegend Cat. No. 161002)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Antigen References

1. Schyns J, et al. 2019. Nat. Commun. 3;10(1):3964
2. Langlet C, et al. 2012. J. Immunol. 188 (4) 1751-1760.

Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 2    Revision Date: 10/28/2021

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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