Purified anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
GL-1_Purified_090707
C57BL/6 mouse splenocytes stained with purified GL-1,followed by anti-rat IgG FITC
  • GL-1_Purified_090707
    C57BL/6 mouse splenocytes stained with purified GL-1,followed by anti-rat IgG FITC
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Cat # Size Price Quantity Check Availability
105001 50 µg $54.00
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105002 500 µg $138.00
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  3. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  4. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  5. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  6. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  7. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  8. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  9. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  10. Zhang P, et al. 2007. J Immunol. 178:474. PubMed
  11. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  12. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  13. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  14. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  15. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  16. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  17. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  18. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  19. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  20. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  21. Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed
  22. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  23. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  24. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  25. Vander B, et al. 2017. J Cell Biol. 10.1083/jcb.201512012. PubMed
  26. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  27. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  28. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  29. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  30. Zhong Z, et al. 2022. Bioact Mater. 10:195. PubMed
  31. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  32. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  33. Klinger M, et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293:R677. PubMed
RRID
AB_313144 (BioLegend Cat. No. 105001)
AB_313144 (BioLegend Cat. No. 105002)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
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