Purified anti-mouse CD90 Antibody

Pricing & Availability
Clone
G7 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy1
Isotype
Rat IgG2c, κ
G7_Pure_042408
C57BL/6 splenocytes stained with purified G7, followed by anti-rat IgG FITC
  • G7_Pure_042408
    C57BL/6 splenocytes stained with purified G7, followed by anti-rat IgG FITC
  • G7_Pure_CD90_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified CD90 clone G7 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Check Availability
105201 50 µg $54.00
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105202 500 µg $191.00
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Description

CD90 is a 25-35 kD immunoglobulin superfamily member, also known as Thy-1. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in costimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45. The G7 antibody is able to stimulate T cell proliferation and IL-2 secretion and induce apoptosis of thymocytes and CTL clones.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse T-cell hybridoma C6/G8
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone G7 binds to Thy-1 epitope region A and also recognizes Thy-1.1/CD90.1 and Thy-1.2/CD90.2 alloantigens. Additional reported applications (for the relevant formats) include: in vitro T-cell activation1,3-4,6, induction of apoptosis2,5, and immunohistochemical staining7,8 of zinc-fixed paraffin-embedded sections and acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105204).

Application References

(PubMed link indicates BioLegend citation)
  1. Gunter KC, et al. 1984. J. Exp. Med. 159:716. (Activ)
  2. Hueber AO, et al. 1994. J. Exp. Med. 179:785. (Apop)
  3. Tentori L, et al. 1988. J. Immunol. 140:1089. (Activ)
  4. Sugiyama E, et al. 1990. Cell. Immunol. 130:271. (Activ)
  5. Ucker DS, et al. 1992. J. Immunol. 149:1583. (Apop)
  6. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Activ)
  7. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  8. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  2. Rafaiee R, et al. 2023. Bioimpacts. 13:97. PubMed
  3. Zhang W, et al. 2021. Cell Prolif. 54:e13058. PubMed
  4. Souza Bomfim GH, et al. 2020. Cell Calcium. 87:102187. PubMed
  5. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  6. Kuwayama R, et al. 2022. Nat Commun. 13:3107. PubMed
  7. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  8. Kwon OJ, et al. 2019. iScience. 13:328. PubMed
RRID
AB_313168 (BioLegend Cat. No. 105201)
AB_313168 (BioLegend Cat. No. 105202)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al.1993 J. Exp. Med. 177:1331.
3. Mayani H, et al. 1994 Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org
Go To Top Version: 3    Revision Date: 10/25/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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