- Clone
- MK10A6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
- Isotype
- Rat IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 521801 | 50 µg | $89.00 | |||
| 521802 | 500 µg | $265.00 | |||
IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon gamma (IFN-γ) synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10, and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNF-α (via TNF receptor p55, NF-κB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFNγ-induced release of IL-1α, IL-6, IL-8, G-CSF, GM-CSF, and TNF-α. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNF-α receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by downregulation of constitutive and IFNγ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86, and of some adhesion molecules such as CD58.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 31.3 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture: The purified MK10A6 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505003) as the detecting antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. No. 431417 & 431418) are specially developed and recommended. - RRID
-
AB_2561713 (BioLegend Cat. No. 521801)
AB_2561713 (BioLegend Cat. No. 521802)
Antigen Details
- Structure
- Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
- Distribution
- IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells.
- Function
- IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes.
- Interaction
- IL-10R is expressed in monocytes, NK, B, and T cells. In addition Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
- Ligand/Receptor
- IL-10 binds to their receptors IL-10R1 and IL-10R2, and initiates a STAT3-dependent signaling cascade.
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fiorentino DF, et al. 1989. J. Exp. Med. 170:2081.
2. Ho AS, et al. 1993. Proc. Natl. Acad. Sci. USA 90:11267.
3. Hart PH, et al. 1996. J. Immunol. 157:3672.
4. Asadullah K, et al. 2003. Pharmacol. Rev. 55:241.
5. Mosser DM and Zhang X. 2008. Immunol. Rev. 226:205.
6. Maynard CL and Weaver CT. 2008. Immunol. Rev. 226:219. - Gene ID
- 16153 View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
Other Formats
View All IL-10 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse IL-10 | MK10A6 | ELISA Capture |
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Purified anti-mouse IL-10
