- Clone
- C18.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-12 p70, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF)
- Isotype
- Rat IgG2a, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 511801 | 50 µg | $94.00 | |||
| 511802 | 500 µg | $305.00 | |||
Interleukin 12 (IL-12) is a heterodimeric pleiotropic 70 kD (p70) cytokine consisting of a 40 kD (p40) subunit and a 35 kD (p35) subunit. IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages in response to pathogens or upon interaction with activated T cells.IL-12 p70 stimulates cytotoxic T, NK and LAK cell proliferation, andinduces IFN-γ and TNF production in resting and activated T and NK cells.It has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO expressed, recombinant mouse IL-12 p70
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard.
- Product Citations
-
- RRID
-
AB_2123771 (BioLegend Cat. No. 511801)
AB_2123771 (BioLegend Cat. No. 511802)
Antigen Details
- Structure
- heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit
- Distribution
- Secreted by dendritic cells, monocytes/macrophages, B cells, T cells
- Function
- Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
- Interaction
- T cells, NK cells
- Ligand/Receptor
- IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35
- Bioactivity
- IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes.
- Cell Type
- Dendritic cells, Monocytes, Macrophages, B cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.
2. Parham C, et al. 2002 J. Immunol.. 168:5699.
3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Quesniaux V. 1992. Research Immunol. 143:385.
6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. - Gene ID
- 16159 View all products for this Gene ID 16160 View all products for this Gene ID
- UniProt
- View information about IL-12 p70 on UniProt.org
Other Formats
View All IL-12 (p70) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse IL-12 (p70) | C18.2 | ELISA Capture |
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Purified anti-mouse IL-12 (p70)
