- Clone
- 10B9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Podocalyxin
- Isotype
- Rat IgG1, κ
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Mouse endothelial cell line, bEnd.3, was stained with purified anti-mouse Podocalyxin (filled histogram) or rat IgG1, κ isotype control (open histogram) followed by anti-rat IgG PE. -
C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. The section was stained with 5 µg/mL purified anti-mouse Podocalyxin followed by anti-mouse IgG Alexa Fluor® 555. Nuclei were counterstained with DAPI (blue). The image was captured by a 20X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 107902 | 100 µg | $317.00 | |||
Podocalyxin is sialomucin, most closely related to CD34 and endoglycan, expressed by kidney podocytes, hematopoietic progenitors, vascular endothelia, and a subset of neurons. It regulates both cell adhesion and cell morphology and plays an essential role in the formation of podocyte foot processes.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- OSM-dependent endothelial-like cell line, LO
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Onitsuka, I., et al., 2010. Gastroenterology, 138:1525-1535.
- Doyonnas, R., et al., 2005. Blood, 105:4170-4178.
- Minegishi, N., et al., 2003. Blood, 102:896-905.
- Minehata, K., et al., 2002. Blood, 99:2360-2368.
- Doyonnas, R., et al., 2001. J. Exp. Med., 194:13-28.
- Hara, T., et al., 1999. Immunity, 11:567-578.
- RRID
-
AB_2904281 (BioLegend Cat. No. 107902)
Antigen Details
- Structure
- Single-pass type I membrane protein, sialoglycoprotein
- Distribution
-
Apical cell membrane, membrane raft
- Function
- Cell adhesion, cell migration
- Interaction
- Forms a complex with EZR and SLC9A3R2.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
- Antigen References
-
- Refaeli I, et al. 2020. Scientific Reports 10:9419
- Economou C, et al. 2004. J Cell Sci (2004) 117 (15): 3281–94.
- Cait J, et al. 2019. Proc Natl Acad Sci. 116 (10) 4518-4527
- Doyonnas R, et al. 2005. Blood. 105(11):4170-8
- Zhang H, et al. 2014. Stem Cells. 32(1):191-203
- Gene ID
- 27205 View all products for this Gene ID
- UniProt
- View information about Podocalyxin on UniProt.org
Other Formats
View All Podocalyxin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Podocalyxin | 10B9 | FC,IHC-F |
| PE anti-mouse Podocalyxin | 10B9 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse Podocalyxin
Mouse endothelial cell line, bEnd.3, was stained with purifi... 
C57BL/6 mouse frozen kidney section was fixed with 4% parafo... -
PE anti-mouse Podocalyxin
bEND.3 cells were stained with anti-mouse Podocalyxin (clone...