Purified anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
H57-597_Pure_071608.jpg
C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_Pure_071608.jpg
    C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_pure_TCR-Beta_Antibody_IHC-F_102518
    Fresh, frozen mouse spleen was stained with purified TCR β chain clone H57-597 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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109201 50 µg $54.00
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109202 500 µg $138.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F  - Verified
IP, Costim, Depletion - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  2. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  3. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  6. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  7. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
  8. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  9. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  10. McDole J, et al. 2010. Am J Pathol. 177:1823. PubMed
  11. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  12. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  13. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  14. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  15. Tan A, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.004. PubMed
  16. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  17. Willimsky G, et al. 2021. Elife. 10:. PubMed
  18. Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
  19. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  20. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  21. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  22. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  23. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  24. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  25. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  26. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  27. Sharba S, et al. 2019. Virulence. 10:97. PubMed
  28. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  29. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  30. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  31. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  32. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  33. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  34. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  35. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  36. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  37. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  38. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  39. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  40. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  41. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  42. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  43. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  44. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  45. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  46. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  47. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  48. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  49. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  50. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  51. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  52. Sugiyarto G, et al. 2021. Immunother Adv. 1:ltaa001. PubMed
  53. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  54. Dubik M, et al. 2021. Front Neurosci. 15:682451. PubMed
  55. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
RRID
AB_313424 (BioLegend Cat. No. 109201)
AB_313424 (BioLegend Cat. No. 109202)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 5    Revision Date: 08/09/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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