- Clone
- 1B3.3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tib, TCRB, TCRbeta
- Isotype
- Armenian Hamster IgG
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C57BL/6 mouse splenocytes were stained with purified anti-mouse TCR vβ8.3 (clone 1B3.3, left) or with purified Armenian Hamster IgG isotype control (right) followed by anti-Armenian Hamster IgG FITC. The cells were then stained with anti-mouse CD3 APC.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 156302 | 500 µg | $229.00 | |||
The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts with Vβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in the periphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci. Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 and selectively eliminate those T cells in vivo.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- 3.L2 T Cell Clone Expressing the vβ8.3 TCR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone 1B3.3 recognizes TCR vß8.3 from both C57BL/6 and BALB/c strains.
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Application References
(PubMed link indicates BioLegend citation) -
- Kersh G, et al. 1998. J. Immunol. 161:585.
- Product Citations
-
- RRID
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AB_2750428 (BioLegend Cat. No. 156302)
Antigen Details
- Structure
- Ig super family; TCR is an 85-kd hetero-dimer composed of alpha and beta chain.
- Distribution
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Expressed in a subset of αβ T cells
- Function
- Recognition of Peptide/MHC, T cell activation
- Interaction
- CD3
- Ligand/Receptor
- Peptide/MHC complex
- Cell Targets
- CD3
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Haskins K, et al. 1984. J. Exp. Med. 160:452
- Davis MM, et al. 1998. Annu Rev Immunol. 16:523
- Kersh GJ, et al. 1998. J Immunol. 161:585.
- Tomonari K. 1996. Immunogenetics. 44:73
- Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vbeta8.3 on UniProt.org
Other Formats
View All TCR vbeta8.3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse TCR vβ8.3 | 1B3.3 | FC |
| PE anti-mouse TCR vβ8.3 | 1B3.3 | FC |
| FITC anti-mouse TCR vβ8.3 | 1B3.3 | FC |
| TotalSeq™-A1139 anti-mouse TCR vβ8.3 | 1B3.3 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse TCR vβ8.3
C57BL/6 mouse splenocytes were stained with purified anti-mo... -
PE anti-mouse TCR vβ8.3
C57BL/6 mouse splenocytes were stained with CD3 APC and anti... -
FITC anti-mouse TCR vβ8.3
C57BL/6 mouse splenocytes were stained with CD3 APC and anti... -
TotalSeq™-A1139 anti-mouse TCR vβ8.3