Purified anti-Myelin Basic Protein Antibody (Previously Covance catalog# SMI-99P)

Pricing & Availability
Clone
SMI 99 (See other available formats)
Regulatory Status
RUO
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Previously
Covance Catalog# SMI-99P
Isotype
Mouse IgG2b, κ
A_SMI99_PURE_Myelin_IHCP_HuBrain20x_100418
IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale Bar: 50 µm
  • A_SMI99_PURE_Myelin_IHCP_HuBrain20x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale Bar: 50 µm
  • B_SMI99_PURE_Myelin_IHCP_HuBrain10x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • C_SMI99_PURE_Myelin_IHCP_MuBrain10x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 50 µm
  • D_SMI99_PURE_Myelin_IHCP_MuBrain40x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • E_SMI99_PURE_Myelin_IHCP_RatBrain40x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • F_SMI99_PURE_Myelin_WB_10ugload_100418
    Western blot of purified anti-Myelin Basic Protein antibody (clone SMI 99). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability
808403 25 µL $112.00
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808401 100 µL $276.00
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808402 500 µL $499.00
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the CNS. MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution + 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.25 – 0.5 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.2 – 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Additional Product Notes

Myelin basic protein exists in multiple isoforms. By WB, clone SMI 99 detects mainly the 21 kD and 28 kD isoforms in the murine brain tissue, and the 21 kD isoform in the human brain tissue.

Application References

(PubMed link indicates BioLegend citation)
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RRID
AB_2564741 (BioLegend Cat. No. 808403)
AB_2564741 (BioLegend Cat. No. 808401)
AB_2564741 (BioLegend Cat. No. 808402)

Antigen Details

Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Gene ID
4155 View all products for this Gene ID 17196 View all products for this Gene ID 24547 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org
Go To Top Version: 6    Revision Date: 02/08/2024

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