Purified anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
P82H9 (See other available formats)
Regulatory Status
RUO
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
P82H9_PURE_Myelin_Basic_Antibody_1_092017
Western blot of anti-Myelin Basic Protein antibody (clone P82H9) and isotype-matched IgG1 control. Blots were incubated with anti-MBP at 1 µg/ml (left) or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20 µg rat brain lysates: 20 µg.
  • P82H9_PURE_Myelin_Basic_Antibody_1_092017
    Western blot of anti-Myelin Basic Protein antibody (clone P82H9) and isotype-matched IgG1 control. Blots were incubated with anti-MBP at 1 µg/ml (left) or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20 µg rat brain lysates: 20 µg.
  • P82H9_PURE_Myelin_Basic_Antibody_2_092017
    IHC staining of anti-MBP antibody (clone P82H9) on formalin-fixed paraffin-embedded human brain. The tissue was incubated with the primary antibody anti-MBP (left) or mouse IgG1 (right) at 5 µg/mL for 24 hours at 4°C. DAB was used for detection followed by hematoxylin counterstaining.
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Cat # Size Price Quantity Check Availability
850901 25 µg $112.00
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850902 100 µg $276.00
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant MBP protein fragment corresponding to amino acid residues 1-197
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For Immunohistochemistry of paraffin-embedded tissues, the suggested use of this reagent is 1.0 - 5.0 µg per ml It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2716122 (BioLegend Cat. No. 850901)
AB_2716122 (BioLegend Cat. No. 850902)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Tissue distribution: Central and peripheral nervous systems
Cellular distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org
Go To Top Version: 0    Revision Date: 09/20/2017

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