- Clone
- BL28592 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MBP, Golli-MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
- Isotype
- Mouse IgG2a, κ
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Western blot of purified anti-Myelin Basic Protein Phospho (Thr98) antibody (clone BL28592). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105); Lane 4: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105; p-Thr98). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 859201 | 25 µg | $106.00 | |||
Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP. Phosphorylation of MBP at Thr98 has been shown to decrease its interaction with the lipid bilayer and lead to the destabilization of the compact myelin sheath.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human MBP when phosphorylated at threonine 98
- Formulation
- PBS, pH7.4 plus 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C
- Application
-
WB - Quality tested
Direct ELISA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Yon M, et al. 1994. J NeuroImmunol. 58(2):121 (ELISA, WB)
- RRID
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AB_2814581 (BioLegend Cat. No. 859201)
Antigen Details
- Structure
- MBP is a 304 amino acid protein with predicted molecular mass of ~33 kD. MPB exists in multiple isoforms with observed molecular masses of ~ 18, 21 and 33 kD.
- Distribution
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Tissue Distribution: Central and peripheral nervous system
Cellular Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular - Function
- Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
- Interaction
- MBP forms homodimer
- Cell Type
- Oligodendrocytes
- Biology Area
- Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Llyasov AA, et al. 2018. Front Neurosci. 12:733
- Muller C, et al. 2013. Front Cell Neurosci. 7:169
- Harauz G, et al. 2013. J Neurochem. 125(3):334
- Gene ID
- 4155 View all products for this Gene ID
- UniProt
- View information about Myelin Basic Protein Phospho Thr98 on UniProt.org
Other Formats
View All Myelin Basic Protein Phospho (Thr98) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Myelin Basic Protein Phospho (Thr98) | BL28592 | WB,Direct ELISA |
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Purified anti-Myelin Basic Protein Phospho (Thr98)
Western blot of purified anti-Myelin Basic Protein Phospho (...