Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody (Previously Covance catalog# SMI-32P)

Pricing & Availability
Clone
SMI 32 (See other available formats)
Regulatory Status
RUO
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Previously
Covance Catalog# SMI-32P
Isotype
Mouse IgG1
1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • 1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
    IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_2_090517
    Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from mouse Thalamus. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_3_090517
    IHC-Fluorescence staining of Formalin Fixed Paraffin Embedded (FFPE) Rat Cerebellum by anti-Neurofilament H (NF-H), Nonphosphorylated Antibody clone SMI 32. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NF-H_Antibody_WB_011218
    Western blot of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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801702 25 µL $112.00
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801701 100 µL $276.00
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Array Tomography, ICC - Reported in the literature, not verified in house
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.

Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.

This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+ uptake.

Additional Product Notes

This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang Q, Martin LJ. 2011. J. Neurosci., 31:2815-27. (ICC) PubMed
  2. Stevens HE, et al. 2010. J. Neurosci. 30:5590-602. (ICC) PubMed
  3. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658-66. (ICC) PubMed  
  4. Redondo J, et al. 2015. Brain Pathol. 25(6):692. (IHC-P) PubMed  
  5. Feng L, et al. 2017. eNeuro. 4(1): 0331-16.2016. (IHC-P) PubMed
  6. Feng L, et al. 2014. Invest Ophthalmol Vis Sci. 54(2): 1106–1117. (IHC-P) PubMed
  7. Theotokis, et al. 2016. J. Neuroinflammation 13(1):265 (IHC-P)
  8. Bennett, et al. 2015. J. Neurosci. Methods 245:25-36 (Array Tomography)
  9. Petzold A, et al. 2011. Brain 134:464. (WB) PubMed  
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Zhou Y, et al. 2019. J Clin Invest. 130:1756. PubMed
  3. Abernathy DG et al. 2017. Cell stem cell. 21(3):332-348 . PubMed
  4. Zhang LY, et al. 2020. Theranostics. 0.468055556. PubMed
  5. Beckman D, et al. 2022. Cell Rep. 41:111573. PubMed
  6. Metzner K, et al. 2022. Front Cell Dev Biol. 10:965382. PubMed
  7. Matsuda K, et al. 2022. Cereb Cortex Commun. 3:tgac046. PubMed
  8. Pinton L, et al. 2023. Nat Protoc. 18:1337. PubMed
  9. Jia J, et al. 2023. Cell Death Dis. 14:156. PubMed
  10. Castellanos-Montiel MJ, et al. 2023. Cells. 12:. PubMed
  11. Pestronk A, et al. 2023. Neuropathol Appl Neurobiol. 49:e12898. PubMed
  12. Schmitt LI, et al. 2023. Acta Neuropathol. 145:611. PubMed
  13. Zahaf A, et al. 2023. Nat Commun. 14:1592. PubMed
  14. Hazra R, et al. 2023. Biomedicines. 11:. PubMed
  15. Chen TH, et al. 2023. Mol Ther Nucleic Acids. 32:144. PubMed
  16. Fiedler T, et al. 2023. J Neuroinflammation. 20:100. PubMed
  17. Del Bondio A, et al. 2023. JCI Insight. 8:. PubMed
  18. Samtani G, et al. 2023. Front Cell Neurosci. 17:1169786. PubMed
  19. Abdelwahab T, et al. 2023. iScience. 26:106698. PubMed
  20. Castelli LM, et al. 2021. Mol Neurodegener. 16:53. PubMed
  21. Tsolias A, et al. 2022. Front Neural Circuits. 15:795325. PubMed
  22. Munot P, et al. 2021. Neuropathol Appl Neurobiol. Online ahead of print. PubMed
  23. Hodgetts SI, et al. 2022. Neural Regen Res. 17:1376. PubMed
  24. Tan H, et al. 2022. J Comp Neurol. 530:1276. PubMed
  25. Gao J, et al. 2022. J Comp Neurol. 530:1494. PubMed
  26. McDonald AJ, et al. 2022. Brain Res. 1777:147767. PubMed
  27. Southam K, et al. 2022. J Physiol. 600:1611. PubMed
  28. Riechers SP, et al. 2022. Mol Metab. 60:101468. PubMed
  29. Caval-Holme FS, et al. 2022. J Neurosci. 42:4101. PubMed
  30. Kowal TJ, et al. 2022. J Comp Neurol. 530:2176. PubMed
  31. Ballester-Rosado CJ, et al. 2022. Ann Neurol. 92:45. PubMed
  32. Cunningham ME 2022. Experimental Neurology. 355:114127. PubMed
  33. Weil T, et al. 2022. Sci Adv. 8:eabn3567. PubMed
  34. Gaja-Capdevila N, et al. 2022. Int J Mol Sci. 23: . PubMed
  35. Januel C, et al. 2022. Cell Mol Life Sci. 79:441. PubMed
  36. Ma C, et al. 2022. J Leukoc Biol. 112:1387. PubMed
  37. Rosas Almanza J, et al. 2022. J Neurosci Res. 100:2213. PubMed
  38. Kapar O, et al. 2022. Neurosurg Focus. 53:E6. PubMed
  39. Gallego-Ortega A, et al. 2022. Front Neuroanat. 16:1054849. PubMed
  40. Zhang Y, et al. 2022. Front Cell Neurosci. 16:1069617. PubMed
  41. Kumar MS, et al. 2022. Int J Mol Sci. 23: . PubMed
  42. Tsitsipatis D, et al. 2022. Aging (Albany NY). 14:9832. PubMed
  43. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  44. Li Z, et al. 2023. J Neuroinflammation. 20:16. PubMed
  45. Kapell H, et al. 2023. J Clin Invest. :. PubMed
  46. Zhang S, et al. 2022. Neuron. 110:992. PubMed
  47. Pellegatta M, et al. 2022. J Neurosci. 42:2433. PubMed
  48. Hagemann C, et al. 2022. Adv Healthc Mater. 11:e2101817. PubMed
  49. Zhu PP, et al. 2022. Hum Mol Genet. 31:2779. PubMed
  50. Stevens H, et al. 2010. J Neurosci. 30:5590-5602. PubMed
  51. Cocozza G, et al. 2018. Brain Behav Immun. 73:584. PubMed
  52. Penndorf D et al. 2017. PloS one. 12(8):e0183684 . PubMed
  53. Ruff T, et al. 2021. Front Mol Neurosci. 14:790466. PubMed
  54. Bakken TE, et al. 2021. Nature. 598:111. PubMed
  55. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  56. Atkinson R, et al. 2021. Dis Model Mech. 14:dmm047548. PubMed
  57. Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed
  58. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  59. Leo M, et al. 2022. Cells. 11:. PubMed
  60. Li X, et al. 2017. Mol Ther. 25(1):140-152. PubMed
  61. Trikamji B, et al. 2021. Muscle Nerve. 63:506. PubMed
  62. Ding B, et al. 2021. J Neurosci. 41:2024. PubMed
  63. Mahajan KR, et al. 2020. Ann Neurol. 88:81. PubMed
  64. Mikhalkin A, et al. 2020. Journal of Comparative Neurology. 529(7):1430-1441. PubMed
  65. Yoo M, Kim T 2016. Sci Rep. 6:28548. PubMed
  66. Corsini S, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.232. PubMed
  67. Wong R, et al. 2019. Brain Behav Immun. 76:126. PubMed
  68. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  69. Drohomyrecky PC, et al. 2019. J Immunol. 203:2588. PubMed
  70. Gaja-Capdevila N, et al. 2021. Front Pharmacol. 12:780588. PubMed
  71. Sainio MT, et al. 2022. Front Cell Dev Biol. 9:820105. PubMed
  72. Shi L, et al. 2021. Immunity. . PubMed
  73. Iida M, et al. 2019. Nat Commun. 10:4262. PubMed
  74. Doust YV, et al. 2021. Front Neurol. 12:722526. PubMed
  75. Utagawa EC, et al. 2022. Acta Neuropathol Commun. 10:86. PubMed
  76. Shi X, et al. 2021. Nat Commun. 12:6943. PubMed
  77. Prukop T, et al. 2020. J Neurosci Res. 1933:98. PubMed
  78. Brodie-Kommit J, et al. 2021. Science Advances. 7(11):. PubMed
  79. Saba L, et al. 2016. Cereb Cortex. 26: 1512-1528. PubMed
  80. Li S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1937 - 1942. PubMed
  81. Reinhard K, et al. 2020. eLife. 8:e50697.. PubMed
  82. Honig MG, et al. 2021. Front Neurosci. 15:701317. PubMed
  83. , et al. 2021. Nature. 598:151. PubMed
  84. Kondo T, et al. 2022. Front Cell Neurosci. 16:858562. PubMed
  85. Harley J, et al. 2021. Brain Commun. 3:fcab166. PubMed
  86. Calvo-Barreiro L, et al. 2021. Neurotherapeutics. . PubMed
  87. Merkulyeva N, et al. 2021. Neuroscience Letters. 762:136165. PubMed
  88. Laug D, et al. 2019. J Clin Invest. 129:4408. PubMed
  89. Takakura K, et al. 2017. Heliyon. 3:e00462. PubMed
  90. Tung YT et al. 2019. Cell Stem Cell. 25(2):193-209 . PubMed
  91. Ondatje BN, et al. 2022. Lab Chip. 22:4246. PubMed
  92. Sharf T, et al. 2022. Nat Commun. 13:4403. PubMed
  93. Mavlyutov TA, et al. 2022. Cell Biosci. 12:72. PubMed
  94. Chen D, et al. 2022. J Neuroinflammation. 19:112. PubMed
  95. Clark CM, et al. 2021. Brain Sci. 11:. PubMed
  96. Simone R, et al. 2021. Nature. 594:117. PubMed
  97. Candadai AA, et al. 2021. PLoS One. e0247901:16. PubMed
  98. Ho R, et al. 2020. Cell Systems. 12(2):159-175.e9. PubMed
  99. Rizzo F, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw258. PubMed
  100. örner S, et al. 2016. J Neuropathol Exp Neurol. 10.1093/jnen/nlw003. PubMed
  101. Yi‐Lan Weng et al. 2018. Neuron. 97(2):313-325 . PubMed
  102. McLeod VM et al. 2019. Br J Pharmacol. 176(13):2111-2130 . PubMed
  103. Schirmer L, et al. 2019. Nature. 573:75. PubMed
  104. Yang C, et al. 2020. Neuron. 105:276. PubMed
  105. Abo-Rady M, et al. 2020. Stem Cell Reports. 14:390. PubMed
  106. Risner ML, et al. 2022. Mol Neurobiol. 59:1366. PubMed
  107. Yu M, et al. 2022. Front Neurol. 13:903565. PubMed
  108. Ou Y, et al. 2016. J Neurosci. 36: 9240 - 9252. PubMed
  109. Himmelein S, et al. 2017. J Virol. 10.1128/JVI.00331-17. PubMed
  110. Miguel JC, et al. 2021. Front Aging Neurosci. 645334:13. PubMed
  111. Saraf MP et al. 2019. The Journal of comparative neurology. 527(15):2599-2611 . PubMed
  112. Ben Haim L, et al. 2021. Glia. 69:2812. PubMed
  113. van der Heijden ME, et al. 2021. J Physiol. 599:2037. PubMed
  114. Jiang D, et al. 2019. Journal of Comparative Neurology. 528(5):729-755. PubMed
  115. Azeez I, et al. 2016. J Neuropathol Exp Neurol. 75: 843 - 854. PubMed
  116. Donkels C, et al. 2016. Cereb Cortex. 10.1093/cercor/bhv346. PubMed
  117. Kinoshita H, et al. 2019. Sci Rep. 9:11519. PubMed
  118. Groh J, et al. 2021. Brain Commun. 3:fcab047. PubMed
  119. Petrozziello T, et al. 2017. Cell Death Differ. 10.1038/cdd.2016.154. PubMed
  120. Chavali M, et al. 2020. Neuron. . PubMed
  121. Roboon J, et al. 2019. Front Cell Neurosci. 13:258. PubMed
  122. Okigawa S, et al. 2021. J Comp Neurol. 529:2099. PubMed
  123. Lanz T, et al. 2017. Sci Rep. 7:41271. PubMed
  124. Brambilla L, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw161. PubMed
  125. Ebert T 2016. Hum Mol Genet. 25: 514 - 523. PubMed
  126. Ito K, et al. 2018. Sci Rep. 33:1052. PubMed
  127. Saraf MP et al. 2018. The Journal of comparative neurology. 527(3):625-639 . PubMed
  128. Hirono M, et al. 2018. J Neurosci. 38:6130. PubMed
  129. Reinehr S, et al. 2019. Int J Mol Sci. 2.613194444. PubMed
  130. Tan H, et al. 2019. Cell Death Differ. 27:1369. PubMed
  131. van der Heijden ME, et al. 2021. Elife. 10:. PubMed
  132. Wolf C, et al. 2022. Commun Biol. 5:541. PubMed
  133. Parisi C, et al. 2016. Cell Death Differ. 23:531-541. PubMed
  134. Wagener R, et al. 2016. Cereb Cortex. 26: 820 - 837. PubMed
  135. Ho R, et al. 2016. Nat Neurosci. 10.1038/nn.4345. PubMed
  136. Hughes RO, et al. 2021. Cell Reports. 34(1):108588. PubMed
  137. Korzhevskii DE, et al. 2017. Zh Nevrol Psikhiatr Im S S Korsakova. 117:50. PubMed
  138. Dyer M, et al. 2019. Front Aging Neurosci. 11:68. PubMed
  139. Larson VA et al. 2018. eLife. 7 pii: e34829. PubMed
  140. Kelley KW et al. 2018. Neuron. 98(2):306-319 . PubMed
  141. Puller C, et al. 2020. J Neurosci. 40:1302. PubMed
  142. Casanovas A, et al. 2017. Sci Rep. 7:40155. PubMed
  143. Berry R, et al. 2015. PLoS One. 10: 0144341. PubMed
  144. Wang H, et al. 2015. Sci Rep. 5: 17383. PubMed
  145. De Pace R, et al. 2018. PLoS Genet. 8:6458. PubMed
  146. Jiang LL, et al. 2019. J Clin Invest. 130. PubMed
  147. Nelke A, et al. 2022. Front Pharmacol. 12:773925. PubMed
  148. Thiry L, et al. 2022. ASN Neuro. 14:17590914211073381. PubMed
  149. Whye D, et al. 2022. Curr Protoc. 2:e568. PubMed
  150. Martin Q 2011. J Neurosci. 31:2815-2827. PubMed
  151. Petzold A, et al. 2011. Brain. 134:464-483. PubMed
  152. Redondo J, et al. 2015. Brain Pathol. 25:692-700. PubMed
  153. Faustino Martins JM, et al. 2020. Cell Stem Cell. 172:26. PubMed
  154. Miao W, et al. 2020. J Immunol. 1486:204. PubMed
  155. Fouda AY, et al. 2020. Invest Ophthalmol Vis Sci. 51:61. PubMed
  156. Cuadrado E et al. 2019. Cell reports. 26(7):1718-1726 . PubMed
  157. Maruyama T, et al. 2018. Cell Death Dis. 12:146. PubMed
  158. Pelisch N, et al. 2021. Eneuro. 8:. PubMed
  159. Swanson OK, et al. 2021. Eneuro. 8:. PubMed
  160. Alpár A, et al. 2018. EMBO J. 37:. PubMed
  161. Alcover-Sanchez B, et al. 2021. Glia. 69:619. PubMed
  162. Sen T, et al. 2020. J Neurosci. 40:424. PubMed
  163. Litvina EY et al. 2017. Neuron. 96(2):330-338 . PubMed
  164. Wang F, et al. 2021. Current Biology. 31(11):2263-2273.e3. PubMed
  165. Bukreeva I, et al. 2017. Sci Rep. 7:41054. PubMed
  166. Feng L, et al. 2017. eNeuro. 4(1). PubMed
  167. MacNair L, et al. 2016. Brain. 139: 86 - 100. PubMed
  168. Wood KC et al. 2017. PloS one. 12(1):e0170264 . PubMed
  169. Abbas Farishta R, et al. 2021. Cereb Cortex Commun. 1:tgaa030. PubMed
  170. Turner M, et al. 2015. J Neuroimmunol. 285: 4-12. PubMed
  171. Pagliarini V, et al. 2015. J Cell Biol. 211: 77 - 90. PubMed
  172. Kiryu-Seo S, et al. 2010. J Neurosci. 30:6658-6666. PubMed
  173. Luna G, et al. 2016. Exp Eye Res. 150: 4-21. PubMed
  174. Roboon J, et al. 2021. Journal of Neurochemistry. . PubMed
  175. Eixarch H, et al. 2020. Neurotherapeutics. 17:1988. PubMed
  176. Griggs WS et al. 2017. Frontiers in neuroanatomy. 0.531944444 . PubMed
  177. Krieger B, et al. 2017. PLoS One. 12:e0180091. PubMed
  178. Granatiero V, et al. 2021. Autophagy. 17:4029. PubMed
  179. Ryan BJ, et al. 2021. J Neurosci. 41:3731. PubMed
  180. Khandker L, et al. 2022. Cell Rep. 38:110423. PubMed
  181. Niu F, et al. 2022. Elife. 11:. PubMed
  182. Henschke JU, et al. 2021. Cell Calcium. 96:102390. PubMed
  183. Richard P, et al. 2020. Autophagy. :1. PubMed
  184. Doroshenko ER, et al. 2021. Front Immunol. 570425:12. PubMed
  185. Wegscheid ML, et al. 2021. Cell Reports. 36(1):109315. PubMed
  186. Salapa HE, et al. 2019. Journal of Neuroscience Research. 98:704. PubMed
  187. Sepehrimanesh M, et al. 2020. American Journal of Physiology-Cell Physiology. 319(4):C771-C780. PubMed
  188. Lv Q, et al. 2021. Cereb Cortex. 31:341. PubMed
RRID
AB_2715852 (BioLegend Cat. No. 801702)
AB_2715852 (BioLegend Cat. No. 801701)

Antigen Details

Structure
Neurofilament H has an apparent molecular mass of 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion

Function
NF-H Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter. Phosphorylation of NF-H results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Receptors
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation result in changes to the neurofilament function.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments, Phospho-Proteins
Antigen References
  1. Turner M, et al. 2015. Journal of Neuroimmunology. 285: 4. PubMed
  2. Pagliarini V, et al. 2015. J. Cell Biol.. 211: 77. PubMed
  3. Petzold A, et al. 2011. Brain 134. (WB) PubMed 
  4. Yuan A, et al. 2016. Brain Res Bull  126(3): 334.
  5. Parlakian A, et al. 2016. Rev Neurol. 172(10): 607.
  6. Li D, et al. 2016. Front Aging Neurosci. 8: 290.
  7. Costa J, et al. 2016. Clin Chim Acta. 455: 7.
  8. Lad SP, et al. 2010.  J Stroke Cerebrovasc Dis. 21(1): 30.
Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

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