- Clone
- W16200A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nuclear factor NF-kappa-B p105/p50 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
- Isotype
- Rat IgG2a, κ
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Total lysates (15 µg protein) from HeLa, Molt-4 and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti- NF-κB p105/p50 antibody (clone W16200A, upper) or GAPDH (clone Poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized using an HRP goat anti-rat-IgG (clone Poly4054, Cat. No. 405405) secondary antibody for NFKB1 or HRP Donkey anti-rabbit IgG (clone Poly4064, Cat. No. 406401) antibody for GAPDH and chemiluminescence detection. Lane M is the MW ladder.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 603901 | 25 µg | $101.00 | |||
| 603902 | 100 µg | $253.00 | |||
NF-κB p105/p50 is the class 1 member of the nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB) transcription factor family. NFKB1 is synthesized as a longer precursor protein, p105, which can be prolytically processed into shorter proteins, p50 and IκBγ. Both p105 and IκBγ proteins contain IκB-like ankyrin repeats that can act as NF-κB inhibitors. P50 protein forms active DNA-binding heterodimers with RelA/NF-κB p65 and c-REL. Stimulation by inflammatory signals, such as TNFα, IL-1, and LPS, lead to the proteosomal degradation of IκBα and result in the translocation of RelA/NF-κB p65:p50 and c-Rel:p50 heterodimers to the nucleus. Subsequently, the nuclear NF-κB subunits bind to target genes and regulate gene transcription. NFKB1 is a key component of the NF-κB signaling pathway and is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, and immune responses.
Product Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse NF-κB p105/p50 recombinant protein (232-431 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:5000 diluted (0.1 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The clone W16200A detects both p105 precursor protein and p50 subunit.
As per the Human Protein Atlas RNA level dataset, Molt-4 cell is expected to have a low level of NF-?B p105/p50 expression.
This clone does not work for IF microscopy application (in-house tested).
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). - RRID
-
AB_2716155 (BioLegend Cat. No. 603901)
AB_2716155 (BioLegend Cat. No. 603902)
Antigen Details
- Structure
- 968 amino acids with a predicted molecular weight of 105 kD. Contains an N-terminal RHD domain, 7 ankyrin repeats, and a C-terminal death domain. Can be processed to a 50kD p50 protein and a 70kD IκBγ protein.
- Distribution
-
Cytoplasm. Translocates to the nucleus when activated.
- Function
- NFKB1 belongs to the NF-κB transcription factor family and participates in the regulation of cell proliferation, differentiation, apoptosis, and immune responses.
- Interaction
- NFKB1 p50 forms heterodimers with NF-κB p65 and c-Rel. Interacts with MAP3K8, DSIPI, SPAG9, UNC5CL, CFLAR, NFKBIE, NFKBIZ, NFKBID, MEN1, and HIF1AN.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lougaris V, et al. 2017. Clin Immunol. 175:99.
2. Cartwright T, et al. 2016. FEBS J. 283:1812.
3. Li SK, et al. 2015. Mol Cell Biol. 35:1619.
4. Voce DJ, et al. 2015. Oncogene. 34:2807.
5. Chang PY, et al. 2006. Cancer Res. 66:6008.
6. Coleman TA, et al. 1993. Mol Cell Biol. 13:3850. - Gene ID
- 4790 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p105/p50 on UniProt.org
Other Formats
View All NF-κB p105/p50 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NF-κB p105/p50 | W16200A | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-NF-κB p105/p50
Total lysates (15 µg protein) from HeLa, Molt-4 and NIH3T3 c...