- Clone
- 4D1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
- Isotype
- Mouse IgG1, κ
-
Total cell lysates (15 µg protein) from Jurkat (Lane 1) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.025 µg/mL (1:20,000 dilution) Purified anti-NF-κB p50 Antibody, clone 4D1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-NF-κB p50 Antibody , or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder. -
A549 cells were unstimulated (left panel) or treated with 20 ng/mL TNF-α (Cat. No. 717904, right panel) for 16 hours. Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL (1:250 dilution) of Purified anti-NF-κB Antibody, clone 4D1, overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 616701 | 25 µg | $101.00 | |||
| 616702 | 100 µg | $218.00 | |||
NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- recombinant full-length human NF-κB p50
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_315855 (BioLegend Cat. No. 616701)
AB_315855 (BioLegend Cat. No. 616702)
Antigen Details
- Structure
- Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
- Distribution
-
Ubiquitously expressed
- Function
- A transcription factor, originally identified as an activator of kappa light chain in B cells
- Interaction
- Interacts with NF-κB p65 and IκB
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review). - Regulation
- NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
- Gene ID
- 4790 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p50 on UniProt.org
Other Formats
View All NF-κB p50 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NF-κB p50 | 4D1 | WB,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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