Purified anti-NMDAR1 Antibody (Previously Covance catalog# MMS-5145)

Pricing & Availability
Clone
N308/48 (See other available formats)
Regulatory Status
RUO
Other Names
GluN1/NR1 glutamate receptor, Glutamate receptor ionotropic, NMDA 1, NMD-R1, neurotransmitter receptor, NMDAR1 receptor C1 cassette, N-methyl-D-aspartate glutamate receptor, glutamate receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1
Previously
Covance Catalog# MMS-5145
Isotype
Mouse IgG1, κ
N30848_Purified_NMDAR1_Antibody_1_030119_updated
Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426303) was used as the detection system. The smaller band at ~75 kD is an MMP-7 cleaved fragment of NMDAR1.
  • N30848_Purified_NMDAR1_Antibody_1_030119_updated
    Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426303) was used as the detection system. The smaller band at ~75 kD is an MMP-7 cleaved fragment of NMDAR1.
  • N30848_Purified_NMDAR1_Antibody_2_030617
    IHC staining of purified anti-NMDAR1 antibody (clone N308/48) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N30848_Purified_NMDAR1_Antibody_3_030617
    IHC staining of purified anti-NMDAR1 antibody (clone N308/48) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability
818602 25 µL $83.00
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818601 100 µL $165.00
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Technical data sheet

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 42-361 (extracellular, N-terminus) of rat GluN1/NR1 glutamate receptor protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg/mL. For immunohistochemistry on paraffin-embedded tissue sections, a concentration of 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Ruden JB, et al. 2021. Front Mol Neurosci. 14:777049. PubMed
RRID
AB_2564822 (BioLegend Cat. No. 818602)
AB_2564822 (BioLegend Cat. No. 818601)

Antigen Details

Structure
Expected MW: 105 kD
Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
24408 View all products for this Gene ID
UniProt
View information about NMDAR1 on UniProt.org
Go To Top Version: 8    Revision Date: 07/09/2024

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