- Clone
- R1JHL (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GluN1, Glutamate [NMDA] receptor subunit zeta-1, NMD-R1, glutamate [NMDA] receptor subunit zeta 1, N-methyl-D-aspartate receptor subunit NR1, N-methyl-D-aspartate receptor channel, subunit zeta-1
- Previously
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Covance Catalog# MMS-501P
- Isotype
- Mouse IgG1, κ
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Western blot of purified anti-NMDAR1 antibody (clone R1JHL). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.3 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 828201 | 15 µg | $370.00 | |||
Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Product Details
- Verified Reactivity
- Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against a fusion protein from aa1-564 of rat NMDA NR1. It is sold as lyophilized TCS.
- Formulation
- Lyophilized. Stored in DMEM + 10% FBS.
- Preparation
- Lyophilized from hybridoma tissue culture media.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.3 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Reconstitute in 50 ul of distilled water. For immunoblot dilute in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 0.1% tween-20) containing 5% nonfat milk and 0.05% NaN3. - Product Citations
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- RRID
-
AB_2564918 (BioLegend Cat. No. 828201)
Antigen Details
- Structure
- Expected MW: 120 kD
- Cell Type
- Glutamatergic Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
-
- Moriyoshi K, et al. 1991. Nature. 354:31.
- Monyer H, et al. 1992. Science. 256:1217.
- Ishii T, et al. 1993. J Biol Chem. 268:2836.
- Stephenson FA. 2001. Curr Drug Targets. 2:233. (IP)
- Louis JV, et al. 2011. Proc Natl Acad Sci U S A. 108:6957. (IHC)
- Gene ID
- 2902 View all products for this Gene ID
- UniProt
- View information about NMDAR1 on UniProt.org
Other Formats
View All NMDAR1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NMDAR1 | R1JHL | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NMDAR1
Western blot of purified anti-NMDAR1 antibody (clone R1JHL)....