- Clone
- Poly28608 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, Nova-1
- Isotype
- Rabbit Polyclonal IgG
-
Western blot of purified anti-NOVA1 antibody (Poly28608). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-NOVA1 antibody (Poly28608) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 860801 | 25 µg | $125.00 | |||
RNA binding-protein Nova-1(NOVA1) is a brain-specific splicing factor well conserved in vertebrates. It was first identified as an antigen in paraneoplastic opsoclonus-myoclonus ataxia (POMA), a disorder related to breast cancer and motor dysfunction. Nova1 binds to a specific sequence cluster in the brain to regulate neuronal pre-mRNA splicing. Nova1-binding sequences are 94% identical in vertebrates, suggesting the binding is sequence dependent. Nova1 null mice die postnatally from motor deficits associated with neuronal cell death in spinal cord and brain stem.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant NOVA1 (Ala295-Gly510) with two N-terminal tags.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Multiple bands observed on WB correspond to different NOVA1 splice variants (1).
- RRID
-
AB_2810742 (BioLegend Cat. No. 860801)
Antigen Details
- Structure
- Human NOVA1 is a 507 amino acid protein with a predicted molecular mass of ~51 kD.
- Distribution
-
Tissue distribution: Brain
Cellular distribution: Nucleus - Function
- NOVA1 regulates RNA-splicing in neurons.
- Interaction
- Interacts with PTBP2.
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience
- Molecular Family
- Microfilaments
- Antigen References
-
- Jelen N, et al. 2007. PLOS Genet. 3(10):e173
- Buckanovich RJ, et al. 1997. Mol Cell Biol. 17(6):3194-201.
- Jensen KB, et al. 2000. Neuron. 2000. 25(2):359-71.
- Gene ID
- 4857 View all products for this Gene ID
- UniProt
- View information about NOVA1 on UniProt.org
Other Formats
View All NOVA1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NOVA1 | Poly28608 | WB,IHC-P |
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