- Clone
- Poly29035 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitogen-activated protein kinase 14, MAP kinase 14, p38alpha Exip, p38 MAP kinase, MAP kinase Mxi2, MAP kinase p38 alpha, CSAID-binding protein, MAX-interacting protein 2, stress-activated protein kinase 2A, p38 mitogen activated protein kinase
- Previously
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Covance Catalog# PRB-510P
- Isotype
- Rabbit Polyclonal IgG
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Figure Legend: IHC staining of purified anti-p38 MAPK Phospho (Thr180/Tyr182) antibody (clone Poly29035) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:10 dilution of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm -
Total cell lysates (15 µg total protein) from Jurkat cells untreated (-) and treated (+) with anisomycin (25 µg/mL for 15 minutes) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-p38 MAPK Phopsho (Thr180/Tyr182) Antibody, clone Poly29035, at a 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Purified anti-p38 MAPK Antibody, clone Poly6224 (Cat. No. 622402), and Direct-Blot™ anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:500 dilution. Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 903501 | 100 µL | $288.00 | |||
The p38 MAP kinase is a stress-activated protein kinase. The p38 MAP kinase is activated by treatment of cells with proinflammatory cytokines (e.g. TNF and IL-1) or by exposure of cells to environmental stress (e.g. UV radiation and osmotic shock). This activation results in the phosphorylation of residues Thr180 and Tyr182. This antibody is specific for the 39 kD p-38 protein phosphorylated at Thr180/Tyr182 It is purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Formulation
- 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA, and 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1 is suggested. For Western blotting, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This product may contain other non-IgG subtypes.
- Product Citations
-
- RRID
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AB_2565031 (BioLegend Cat. No. 903501)
Antigen Details
- Structure
- p38 MAPK is a with a predicted molecular weight of 39 kD
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Phospho-Proteins, Presynaptic proteins
- Gene ID
- 1432 View all products for this Gene ID
- UniProt
- View information about p38 MAPK Phospho Thr180/Tyr182 on UniProt.org
Other Formats
View All p38 MAPK Phospho (Thr180/Tyr182) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-p38 MAPK Phospho (Thr180/Tyr182) | Poly29035 | IHC-P,WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-p38 MAPK Phospho (Thr180/Tyr182)
Figure Legend: IHC staining of purified anti-p38 MAPK Phosph... 
Total cell lysates (15 µg total protein) from Jurkat cells u...