Purified anti-p62 (SQSTM1) Antibody (Previously Covance catalog# MMS-5034)

Pricing & Availability
Clone
1B5.H9 (See other available formats)
Regulatory Status
RUO
Other Names
P60, p62, A170, OSIL, PDB3, ZIP3, p62B, sequestosome-1, oxidative stress-induced-like ubiquitin-binding protein p62
Previously
Covance Catalog# MMS-5034
Isotype
Mouse IgG1, κ
1B5dotH9_Pure_p62_Antibody_1_102318
IHC staining of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 15 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1B5dotH9_Pure_p62_Antibody_1_102318
    IHC staining of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 15 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1B5dotH9_Pure_p62_Antibody_2_102318
    IHC staining of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 15 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1B5dotH9_Pure_p62_Antibody_3_102318
    Western blot of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293 cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1B5dotH9_Pure_p62_Antibody_3-5_102721
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 50 µM chloroquine phosphate (Cat. No. 427502) for 18 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 1B5dotH9_Pure_p62_Antibody_4_102318
    ICC staining of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) on A549 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1B5dotH9_Pure_p62_Antibody_5_102318
    ICC staining of purified anti-p62 (SQSTM1) antibody (clone 1B5.H9) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability
814802 25 µL $112.00
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814801 100 µL $275.00
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Description

The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein that colocalizes with ubiquitinated protein aggregates in many neurodegenerative diseases and proteinopathies of the liver. The protein is able to polymerize via an N-terminal PB1 domain and can interact with ubiquitinated proteins via the C-terminal UBA domain. Also, p62/SQSTM1 binds directly to LC3 and GABARAP family proteins via a specific sequence motif. The protein is itself degraded by autophagy and may serve to link ubiquitinated proteins to the autophagic machinery to enable their degradation in the lysosome. Recent studies have revealed a novel function for p62 in innate immunity.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence corresponding to amino acids 201-212 of the human SQSTM 1 (isoform 1) protein conjugated to KLH.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 10 - 15 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), immunoblotting (WB), and immunocytochemistry (ICC).

Product Citations
  1. Zhou B, et al. 2020. Nat Microbiol. 5:1576. PubMed
  2. Tapia D, et al. 2019. Nat Commun. 10:735. PubMed
  3. Trejo-Lopez JA, et al. 2020. J Neuropathol Exp Neurol. 407:79. PubMed
  4. Quach C, et al. 2019. Nat Commun. 4.361805556. PubMed
RRID
AB_2564787 (BioLegend Cat. No. 814802)
AB_2564787 (BioLegend Cat. No. 814801)

Antigen Details

Structure
p62 (SQSTM1) is a 440 amino acid protein with an expected molecular mass of 62 kD.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Adaptor Proteins, Autophagosome Markers
Gene ID
8878 View all products for this Gene ID
UniProt
View information about p62 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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