- Clone
- Poly28610 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PRDX6, peroxiredoxin VI, Peroxiredoxin-6, AOP2, Antioxidant protein 2
- Isotype
- Rabbit Polyclonal IgG
-
Western blot of purified anti-Peroxiredoxin 6 antibody (Poly28610). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Peroxiredoxin 6 antibody (Poly28610) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-Peroxiredoxin 6 antibody (Poly28610) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 861001 | 25 µg | $125.00 | |||
Human peroxiredoxin 6 is encoded by the PRDX6 gene and belongs to one of six peroxiredoxin families including PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, and PRDX6. Peroxiredoxins are expressed ubiquitously and serve as thiol-specific antioxidant enzymes that regulate the redox state, and reduce H2O2 and lipid peroxides. Peroxiredoxin 6 interacts with saitohin (STH) which is nested within the intron 9 of the Tau gene demonstrates similar protein expression pattern as Tau protein. This interaction combined with abnormal expression of peroxiredoxin 6 may be a contributing factor to several neurodegenerative diseases including Alzheimer’s and Pick’s diseases. In Alzheimer's disease, peroxiredoxin 6 is upregulated in reactive astrocytes which are found colocalized with amyloid-beta (Aβ) peptide-positive diffuse plaques. This upregulation is believed to reduce oxidative stress caused by Aβ neurotoxicity.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant human PRDX6 (amino acid sequence Met1~Pro224)
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody also detects peroxiredoxin 6 dimers in the liver lysate.
- RRID
-
AB_2814585 (BioLegend Cat. No. 861001)
Antigen Details
- Structure
- Peroxiredoxin 6 is a 224 amino acid protein with predicted and observed molecular mass of ~25 kD.
- Distribution
-
Tissue Distribution: Predominantly expressed in the lung, brain, liver, and testis
Cellular Distribution: Nucleus, cytosol, extracellular, lysosome, and plasma membrane - Function
- Peroxiredoxin 6 is involved in redox regulation and protects cells against lipid peroxidation.
- Interaction
- Peroxiredoxin 6 forms homodimer and heterodimer, and interacts with GSTP1, APEX1, and STH.
- Cell Type
- Astrocytes, Mature Neurons, Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- APP/Aβ Degradation, Enzymes and Regulators, Lysosomal Markers
- Antigen References
-
- Manevich Y, et al. 2014. Free Radic Biol Med. 72: 210.
- Zhou S, et al. 2016. Free Radic Biol Med. 94:145.
- Power JH, et al. 2008. Acta Neuropathol. 115:611.
- Gene ID
- 9588 View all products for this Gene ID
- UniProt
- View information about Peroxiredoxin 6 on UniProt.org
Other Formats
View All Peroxiredoxin 6 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Peroxiredoxin 6 | Poly28610 | WB,IHC-P |
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