- Clone
- ATM-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- OX-40 Ligand, CD134L, TNFSF4
- Isotype
- Mouse IgG1, κ
-
PMA-stimulated (2 days) Lou rat splenocytes stained with ATM-2 biotin, then detected with Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 200501 | 50 µg | $94.00 | |||
OX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4), also known as CD252 or CD134L. It is expressed on dendritic cells, peritoneal macrophages, and activated B cells. The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation. The ATM-2 antibody is able to block the costimulatory function of OX-40L.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- ACI-S1 rat T-cell lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).
-
Application References
(PubMed link indicates BioLegend citation) -
- Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
- RRID
-
AB_2571881 (BioLegend Cat. No. 200501)
Antigen Details
- Structure
- TNF superfamily, 22 kD
- Distribution
- Dendritic cells, activated B cells
- Function
- T cell dependent humoral responses
- Ligand/Receptor
- OX40 (CD134)
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.
3. Akiba, H., et al. 2000. J. Exp. Med. 191:375. - Gene ID
- 22164 View all products for this Gene ID
- UniProt
- View information about CD252 on UniProt.org
Other Formats
View All CD252 (OX-40 Ligand) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat CD252 (OX-40 Ligand) | ATM-2 | FC |
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Purified anti-rat CD252 (OX-40 Ligand)
PMA-stimulated (2 days) Lou rat splenocytes stained with ATM...