- Clone
- A20087F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nucleocapsid phosphoprotein, N, Nucleoprotein
- Isotype
- Mouse IgG2b, κ
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Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Recombinant nucleocapsid protein coated onto Costar™ 96-well, high binding assay plate at 2 µg/mL was incubated with a dilution series of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F). Bound antibodies were detected with HRP-goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 940901 | 25 µg | $112.00 | |||
| 940902 | 100 µg | $335.00 | |||
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.
The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection. N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SARS-CoV-2 nucleocapsid recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For the Direct ELISA application, a concentration of 2.5 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) with SARS-CoV has not been tested.
- RRID
-
AB_2888743 (BioLegend Cat. No. 940901)
AB_2888743 (BioLegend Cat. No. 940902)
Antigen Details
- Structure
- SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD.
- Distribution
-
Viral nucleoprotein, host cell Golgi apparatus
- Function
- RNA binding, transcription regulation
- Biology Area
- COVID-19
- Antigen References
-
- Zeng W, et al. 2020. Biochem Biophys Res Commun. 527:618-623.
- Yoshimoto FK. 2020. Protein J. 39:198-216.
- Cascarina SM & Ross ED. 2020. FASEB J. fj.202001351.
- Gene ID
- 43740575 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Other Formats
View All Viral Protein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SARS-CoV-2 Nucleocapsid | A20087F | WB,Direct ELISA |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SARS-CoV-2 Nucleocapsid
Whole cell extracts (15 µg total protein) from 293E cells un... 
Recombinant nucleocapsid protein coated onto Costar™ 96-well...