- Clone
- A20087H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nucleocapsid phosphoprotein, N, Nucleoprotein
- Isotype
- Mouse IgG2b, κ
-
Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Recombinant nucleocapsid protein coated onto Costar™ 96-well, high binding assay plate at 2 µg/mL was incubated with a dilution series of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087H). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm. -
His-tagged SARS-CoV-2 Nucleocapsid transfected CHO cells were stained with anti-Nucleocapsid (clone A20087H) purified (left) or mouse IgG2b, κ isotype control (clone MPC-11) purified (right) followed by anti-mouse IgG2b PE (clone RMG2b-1) and APC anti-His Tag (clone J095G46).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 946101 | 25 µg | $112.00 | |||
| 946102 | 100 µg | $335.00 | |||
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.
The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection. N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SARS-CoV-2 Nucleocapsid recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested.
BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H- Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application
- Both clones have been verified for direct ELISA and displayed similar EC50 values for this application
- A20087H has been verified for ICFC, while A20087F is not recommended for this application
- RRID
-
AB_2892515 (BioLegend Cat. No. 946101)
AB_2892515 (BioLegend Cat. No. 946102)
Antigen Details
- Structure
- SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
- Distribution
-
Viral nucleoprotein, host cell Golgi apparatus
- Function
- RNA binding, transcription regulation
- Antigen References
-
- Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623.
- Yoshimoto FK. 2020. Protein J. 39(3):198-216.
- Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
- Gene ID
- 43740575 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Other Formats
View All Viral Protein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SARS-CoV-2 Nucleocapsid | A20087H | WB,ELISA,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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