- Clone
- A20103H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spike glycoprotein, Sglycoprotein (E2)
- Isotype
- Rat IgG2b, κ
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Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with a plasmid encoding SARS-CoV-2 S protein S1 (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution and Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
200 ng of recombinant SARS-CoV-2 S protein S1 + S2 (Cat. No. 793704) was coated onto a Costar™ 96-well high binding assay plate and incubated with a dilution series of purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103H). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 944401 | 25 µg | $112.00 | |||
| 944402 | 100 µg | $335.00 | |||
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant protein corresponding to the S1 subunit of SARS-CoV-2 S protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ELISA - Verfied - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For ELISA applications, a concentration of 65.4 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.
- RRID
-
AB_2890872 (BioLegend Cat. No. 944401)
AB_2890872 (BioLegend Cat. No. 944402)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
- Distribution
-
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Host cell surface receptor binding
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
-
- Walls AC, et al. 2020. Cell. 181:281-292.
- Yan R, et al. 2020. Science. 367:1444-1448.
- Wrapp D, et al. 2020. Science. 367:1260-1263.
- Shang J, et al. 2020. Proc Natl Acad Sci U S A. 117:11727-11734.
- Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Other Formats
View All Viral Protein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SARS-CoV-2 S Protein S1 | A20103H | WB,ELISA |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SARS-CoV-2 S Protein S1
Whole cell extracts (15 µg total protein) from 293E cells un... 
200 ng of recombinant SARS-CoV-2 S protein S1 + S2 (Cat. No....