- Clone
- AM004414 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spike glycoprotein, S glycoprotein (E2)
- Isotype
- Human IgG1, κ
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A. Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of His-tagged recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792906) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 S Protein S1 recombinant antibody (clone AM004414) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-human IgG antibody at a 1:3000 dilution. Purified anti-His Tag antibody (clone J095G46) was used as a control antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792904) coated onto Nunc™ Maxisorp™ ELISA Plates (Cat. No. 423501) at 2 µg/mL and then incubated with a dilution series of purified anti-SARS-CoV-2 S Protein S1 recombinant antibody (clone AM004414). Bound antibodies were detected with biotinylated anti-human IgG secondary antibody followed by avidin-horseradish peroxidase and TMB substrate solution. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 940401 | 25 µg | $171.00 | |||
| 940402 | 100 µg | $581.00 | |||
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.
This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Recombinant
- Host Species
- Human
- Immunogen
- SARS-CoV-2
- Formulation
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.
- Application
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WB - Quality tested
Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. Recommended usage concentration for direct ELISA application is 93.6 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone name AM004414 is also referred to as clone 414-4.
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Application References
(PubMed link indicates BioLegend citation) -
1. Wan J. et al. 2020. Cell Rep. 32(3):107918
- RRID
-
AB_2888747 (BioLegend Cat. No. 940401)
AB_2888747 (BioLegend Cat. No. 940402)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
- Distribution
-
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Host cell surface receptor binding
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
-
1. Walls A.C. et al. 2020. Cell 181(2):281-292
2. Yan R. et al. 2020 Science 367 (6485):1444-1448
3. Wrapp D. et al. 2020 Science 367 (6483):1260-126
4. Shang J.et al. 2020 PNAS 117(21):11727-11734 - Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Other Formats
View All Viral Protein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody | AM004414 | WB,ELISA |
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Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody
A. Whole cell extracts (15 µg total protein) from HeLa cell... 
Recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792904) coated...