- Clone
- 1A9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SH2 Domain Containing 1A, SAP, T-Cell Signal Transduction Molecule SAP, SLAM-Associated Protein, T Cell Signal Transduction Molecule SAP, MTCP1, XLPD
- Isotype
- Rat IgG2a, κ
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Total cell lysates (15 µg protein) from Jurkat (lane 1), mouse thymus (lane 2) and HeLa (lane 3, negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-SH2D1A (clone 1A9) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 690702 | 100 µg | $253.00 | |||
SH2D1A, also known as SAP, is a small adaptor protein which plays a major role in the bidirectional stimulation of T and B cells. SH2D1A is predominantly expressed in T cells and NK cells, and functions to regulate signal transduction pathways downstream of the SLAM family of surface receptors. The SH2D1A gene is frequently mutated in patients with X-linked lymphoproliferative disease. Malfunction of the SLAM-SAP pathway results in autoimmune diseases, tumors of the lymphoid tissues and inadequate responses to infectious agents.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length purified human SH2D1A.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2629824 (BioLegend Cat. No. 690702)
Antigen Details
- Structure
- 128 amino acids with a characteristic of SH2 domain. The predicted molecular weight is approximately 14kD.
- Distribution
- T cells, NK cells and monocytes.
- Function
- Stimulation of T and B cells.
- Interaction
- SLAM family, SH2D1B, and FYN.
- Ligand/Receptor
- SLAM family; SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7.
- Cell Type
- T cells, NK cells, Monocytes
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Tangye SG. 2014. J. Clin. Immunol. 34:772.
2. Yang X, et al. 2012. Pediatr. Int. 54:447.
3. Ma CS, Deenick EK. 2011. Ann. N Y Acad. Sci. 1217:32-44.
4. Detre C, et al. 2010. Semin. Immunopathol. 32:157.
5. Vilar ML, et al. 2011. Braz. J. Med. Biol. Res. 44:276. - Gene ID
- 4068 View all products for this Gene ID
- UniProt
- View information about SH2D1A on UniProt.org
Other Formats
View All SH2D1A (SAP) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SH2D1A (SAP) | 1A9 | WB |
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Purified anti-SH2D1A (SAP)
Total cell lysates (15 µg protein) from Jurkat (lane 1...