- Clone
- H76 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
- Isotype
- Mouse IgG2a, κ
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Total cell lysates (15 µg total protein) from HeLa (negative control), Daudi (negative control), THP-1, and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SLP-76 antibody, clone H76, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405301) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
Whole cell extracts (15 µg protein) from serum starved Jurkat and EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-SLP76 Phospho (Ser376), clone A18001A (Cat. No. 667102), for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405301) at a 1:3000 dilution. Equal SLP76 loading was confirmed by probing membranes with anti-pan SLP76 antibody, clone H76 (Cat. No. 625002) at a 1:1000 dilution. Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 625002 | 200 µL | $293.00 | |||
SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- amino acids 216-434 of human SLP-76
- Formulation
-
This antibody is provided in phosphate-buffered solution, containing 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, aliquot and store frozen at -20°C. Avoid freeze-thaw cycles.
- Application
-
WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
The 200 µL size can be used for approximately 40 Western blots.
-
Application References
(PubMed link indicates BioLegend citation) -
- Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.
- Product Citations
-
- RRID
-
SCR_001134 (BioLegend Cat. No. 625002)
Antigen Details
- Structure
- Phosphoprotein 76 kD containing SH2 domain in the C-terminus
- Distribution
-
Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.
- Function
- Adaptor molecule involved in B cell receptor and T cell receptor signaling
- Interaction
- Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
- Modification
- Phosphorylation (Y113, Y128, Y145, Y423, Y426)
- Cell Type
- B cells, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029.
2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641.
3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161.
4. Motto DG, et al. 1996. J. Exp. Med. 183:1937. - Gene ID
- 3937 View all products for this Gene ID
- UniProt
- View information about SLP-76 on UniProt.org
Other Formats
View All SLP-76 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SLP-76 | H76 | WB,IP |
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