- Clone
- A18001A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein
- Isotype
- Mouse IgG1, κ
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Whole cell extracts (15 µg protein) from serum starved Jurkat and EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-SLP76 Phospho (Ser376), clone A18001A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal SLP76 loading was confirmed by probing membranes with anti-pan SLP76 Antibody, clone H76 (Cat. No. 625002) at a 1:1000 dilution. Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 667101 | 25 µg | $112.00 | |||
| 667102 | 100 µg | $265.00 | |||
SLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being tranlocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1. SLP76 also plays an essential role in normal T cell development.
SLP76 also represents a negative regulatory node in the TCR activation pathway. Following TCR engagement, SLP76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase (HPK1). This modification promotes an interaction between SLP76 and the scaffolding protein 14-3-3ε, resulting in dissociation of the TCR signaling complex and a diminution of TCR signaling.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic human SLP76 peptide containing phosphorylated Ser376
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone was tested for ICC on PFA-fixed and methanol permeabilized Jurkat cells treated with or without H2O2 and produced nuclear staining that was not responsive to the treatment. We therefore do not recommend this clone for ICC.
†cross-reactivity to Mouse confirmed based on Western blot testing. - RRID
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AB_2801128 (BioLegend Cat. No. 667101)
AB_2801128 (BioLegend Cat. No. 667102)
Antigen Details
- Structure
- SLP76 is a 533 amino acid protein with a predicted molecular weight of ~60 kDa.
- Distribution
-
Hematopoietic cells/cytosol
- Function
- Adapter protein/TCR Signaling
- Interaction
- ITK, LAT, GRB2, PLCγ1, NCK, VAV, ITK, LCK, 14-3-3ε
- Cell Type
- B cells, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Sauer K, et al. 2001. J Biol Chem. 276:45207.
- Koretzky GA, et al.. 2006. Nat Rev Immunol. 6:67.
- Di Bartolo V, et al. 2007. J Exp Med. 204:681.
- Lasserre, et al. 2011. J Cell Biol. 195:839.
- Gene ID
- 3937 View all products for this Gene ID
- UniProt
- View information about SLP76 Phospho Ser376 on UniProt.org
Other Formats
View All SLP76 Phospho (Ser376) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SLP76 Phospho (Ser376) | A18001A | WB |
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Purified anti-SLP76 Phospho (Ser376)
Whole cell extracts (15 µg protein) from serum starved Jurka...