Purified anti-SSRP1 Antibody

Pricing & Availability
Clone
10D1 (See other available formats)
Regulatory Status
RUO
Other Names
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Isotype
Mouse IgG2b, κ
1_10D1_PURE_SSRP1_Antibody_031319
Total cell lysates (15 µg total protein) from THP-1, HaCat, Alexa Fluor® 594 and NTERA2 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of purified anti-SSRP1 antibody, clone 10D1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_10D1_PURE_SSRP1_Antibody_031319
    Total cell lysates (15 µg total protein) from THP-1, HaCat, Alexa Fluor® 594 and NTERA2 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of purified anti-SSRP1 antibody, clone 10D1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_10D1_PURE_SSRP1_Antibody_1_WB_070616
    Total lysates (15 µg protein) from HeLa (lane 1) and NIH3T3 cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mLPurified anti-SSRP1 Antibody, clone 10D1 (upper) or 1:3000 diluted Purified anti-β-actin Antibody, clone Poly6221 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-SSRP1 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-β-actin Antibody.
  • 3_10D1_PURE_SSRP1_Antibody_2_ICC_042219
    HeLa cells were stained with purified anti- SSRP-1 (10D1) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
  • 4_10D1_GoChIP_SSRP1_Antibody_1_052118.PNG
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HeLa cells (starved overnight then treated with 10% FCS) with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-SSRP1 Antibody (clone 10D1, Cat. No. 609709) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # Size Price Quantity Check Availability
609701 25 µg $101.00
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609702 100 µg $218.00
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Description

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D1 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial) (308-524aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For ChIP applications, the suggested dilution is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

10D1 displayed a stronger affinity for SSRP1 than BioLegend clone 3E4 when tested for western blot application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan BC, et al. 2006. EMBO 25:3975. (ChIP)
  2. Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed
  3. Tan BC, et al. 2004. J. Biol. Chem. 279:9321. (Immunogen)
  4. Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed
  5. Aida M, et al. 2013. PNAS. 110:7784. PubMed
  6. Dermawan JK, et al. 2014. Mol Cancer Ther. 13:2203. PubMed
  7. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
Product Citations
  1. Goswami I, et al. 2022. EMBO Rep. 23:e53684. PubMed
  2. Jeong E, et al. 2022. Autophagy. 18:2333. PubMed
  3. Srinivasan R, et al. 2020. Sci Adv. 6:eaaz9115. PubMed
  4. Dermawan J, et al. 2016. Cancer Res . 76: 2432-2442. PubMed
  5. Fleyshman D, et al. 2017. Oncotarget. 8:20525. PubMed
  6. Isa NF, et al. 2021. Vaccines (Basel). 9:. PubMed
  7. Safina A, et al. 2017. Nucleic Acids Res. 10.1093/nar/gkw1366. PubMed
  8. Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed
  9. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
  10. Li X, et al. 2021. J Biol Chem. 297:101360. PubMed
  11. Wever V, et al. 2012. PLoS One. 7:e39510. PubMed
  12. Sundarraj J, et al. 2021. Wellcome Open Res. 2:83. PubMed
  13. Narain A, et al. 2021. Molecular Cell. 81(15):3110-3127.e14. PubMed
  14. Lolis A, et al. 2013. J Biol Chem. 288:7676. PubMed
  15. Piquet S et al. 2018. Molecular cell. 72(5):888-901 . PubMed
  16. Sandlesh P, et al. 2018. PLoS One. 15:81. PubMed
  17. Ali A, et al. 2019. FASEB J. 33:2621. PubMed
  18. Prendergast L, et al. 2020. Oncogene. 39:5124. PubMed
  19. Aida M, et al. 2013. Proc Natl Acad Sci U S A. 110:7784. PubMed
  20. Dermawan J, et al. 2014. Mol Cancer Ther. 13:2203. PubMed
  21. Paul S, et al. 2018. J Biol Chem. 293:19303. PubMed
  22. Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
RRID
AB_315730 (BioLegend Cat. No. 609701)
AB_315730 (BioLegend Cat. No. 609702)

Antigen Details

Structure
SSRP family, HMG box domain; 81 kD
Distribution

Nuclear

Function
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction
Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.

Gene ID
6749 View all products for this Gene ID
UniProt
View information about SSRP1 on UniProt.org
Go To Top Version: 9    Revision Date: 08/24/2021

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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