- Clone
- 3E4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
- Isotype
- Mouse IgG1, κ
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Total cell lysates (15 µg total protein) from NTERA-2, HaCat, and THP-1 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SSRP1 Antibody, clone 3E4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
15 µg of total protein extract from indicated cells was resolved by 4-20% Tris-glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with anti-SSRP1 (clone 3E4) antibody. Purified anti-β-actin (clone Poly6221) antibody was used as loading control. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. -
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-SSRP1 (clone 3E4) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 609802 | 100 µg | $218.00 | |||
SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 3E4 monoclonal antibody recognizes human SSRP1 and has been shown to be useful for Western blotting.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant (partial)
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
-
Application References
(PubMed link indicates BioLegend citation) -
- Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
- Product Citations
-
- RRID
-
AB_2195751 (BioLegend Cat. No. 609802)
Antigen Details
- Structure
- SSRP family, HMG box domain; 81 kD
- Distribution
-
Nuclear
- Function
- Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
- Interaction
- Component of FACT ATP-dependent chromatin remodeling complex, Spt16/Cdc68 in FACT complex, H2A/H2B dimers
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
- Antigen References
-
1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284. - Gene ID
- 6749 View all products for this Gene ID
- UniProt
- View information about SSRP1 on UniProt.org
Other Formats
View All SSRP1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SSRP1 | 3E4 | WB,ICC,IP |
| Alexa Fluor® 647 anti-SSRP1 | 3E4 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SSRP1
Total cell lysates (15 µg total protein) from NTERA-2, HaCat... 
15 µg of total protein extract from indicated cells was reso... 
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Alexa Fluor® 647 anti-SSRP1
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten...