- Clone
- W15088A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
- Isotype
- Mouse IgG2b, κ
-
Total cell lysates (15 µg protein) from Jurkat, 293T and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-STAT2 antibody (clone W15088A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. -
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin. -
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (Clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 693302 | 100 µg | $253.00 | |||
STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT1, p48, and CREB binding protein.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant protein of human STAT2 (671-851 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ChIP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50 - 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W15088A does not cross-react with mouse (in-house tested).
- RRID
-
AB_2632834 (BioLegend Cat. No. 693302)
Antigen Details
- Structure
- STAT family, contains coiled coil and SH2 domain, 113 kD.
- Distribution
-
Translocates into nucleus after phosphorylation. Expressed in skin, thymus.
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT.
- Interaction
- STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
1. Bluyssen HA, Levy DE. 1997. J. Biol. Chem. 272:4600.
2. Qureshi SA, et al. 1996. Mol. Cell. Biol. 16:288. - Gene ID
- 6773 View all products for this Gene ID
- UniProt
- View information about STAT2 on UniProt.org
Other Formats
View All STAT2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-STAT2 | W15088A | WB,ChIP |
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