- Clone
- W21151B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 2, STAT113, Interferon alpha induced transcriptional activator, p113
- Isotype
- Mouse IgG2a, κ
-
Whole cell extracts (15 µg total protein) from serum starved A431 cells untreated (-) or treated (+) with IFN-α (10 ng/mL, 30 min) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-STAT2 phospho (Tyr690) (clone W21151B) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Equal STAT2 loading was confirmed by probing membranes with anti-pan STAT2 antibody. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 619851 | 25 µg | $135.00 | |||
| 619852 | 100 µg | $375.00 | |||
STAT2 is a member of the STAT family of transcription factors that is phosphorylated by receptor associated kinases in response to cytokines and growth factors. After activation, STAT2 forms heterodimers with STAT1. Once it is activated, STAT1 and STAT2 dimerize and are translocated to the nucleus to act as a transcription factor. STAT2 is an important mediator of type I interferon signaling; thus, after type-I IFN binds to the receptor, JAK kinases phosphorylate STAT2 and STAT1. This leads to their dimerization and, together with IRF9/ISGF3G, form the ISGF3 transcription factor. Type-I IFN /STAT2 signaling is important in innate immunity antiviral responses. Additionally, STAT2 is a regulator of mitochondrial fission by modulating phosphorylation of DNM1L. Diseases associated with STAT2 are the Immunodeficiency 44 and Pseudo-Torch Syndrome 3.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human STAT2 phosphorylated at Tyr690
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3068194 (BioLegend Cat. No. 619851)
AB_3068194 (BioLegend Cat. No. 619852)
Antigen Details
- Structure
- STAT2 is an 851 amino acids length protein with a predicted molecular weight of 97.9 kD. STAT2 contains a SH2 domain
- Distribution
-
Cytoplasm/Nucleus
- Function
- Transcription factor, activation in depending of phosphorylation
- Interaction
- Interacts with IRF9/ISGF3G to form ISGF3 transcription factor complex
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins
- Antigen References
-
- Ho J, et al. 2016. PLoS Biol. 14:e2000117.
- Fu X, et al. 1992. PNAS. 89:7840-3.
- Yu w, et al. 2020. Redox Biol. 37:101761
- Lee CJ, et al. 2020. Exp Mol Med. 52:1526-1536.
- Gene ID
- 6773 View all products for this Gene ID
- UniProt
- View information about STAT2 on UniProt.org
Other Formats
View All STAT2 Phospho (Tyr690) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-STAT2 Phospho (Tyr690) | W21151B | WB |
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Purified anti-STAT2 Phospho (Tyr690)
Whole cell extracts (15 µg total protein) from serum starved...