Purified anti-STAT3 Antibody

Pricing & Availability
Clone
4G4B45 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
1_4G4B45_PURE_STAT3_Antibody_WB_110117
Total lysates (15 µg protein) from HeLa and HeLa STAT3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-STAT3 antibody , clone 4G4B45 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 1_4G4B45_PURE_STAT3_Antibody_WB_110117
    Total lysates (15 µg protein) from HeLa and HeLa STAT3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-STAT3 antibody , clone 4G4B45 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 2_4G4B45_PURE_STAT3_Antibody_2_071116
    Total lysates (15 µg protein) from Jurkat (lane 1), PC3 (lane 2, negative control), and NIH3T3 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-STAT3 Antibody, clone 4G4B45 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-STAT3 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 3_4G4B45_PURE_STAT3_Antibody_1_WB_071816
    Hela cells (filled histogram) or PC-3 cells (open histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with purified STAT-3 (clone 4G4B45), followed by anti-mouse IgG PE.
  • 4-4G4B45_PURE_STAT3_Antibody_ICC_110117
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-STAT3 (clone 4G4B45) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 5-4G4B45_PURE_STAT3_Antibody_IP_110117
    Immunoprecipitation of STAT3 from Hela cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-STAT3 antibody (clone 4G4B45, lane 4). Western blot was performed using anti-STAT3 antibody (clone 4G4B45). The astrostar indicates immunoglobulin heavy chain and light chain.
Compare all formats
Cat # Size Price Quantity Check Availability
678001 25 µg $112.00
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678002 100 µg $265.00
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Description

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC, IP, KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Jafari S, et al. 2023. Bioimpacts. 13:51. PubMed
  2. Peng HY, et al. 2023. Signal Transduct Target Ther. 415:8. PubMed
  3. Jafari S, et al. 2020. Daru. 28:159. PubMed
  4. Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed
RRID
AB_2861053 (BioLegend Cat. No. 678001)
AB_2861053 (BioLegend Cat. No. 678002)

Antigen Details

Structure
770 amino acids with a predicted molecular weight of 88.1 kD that contains a C-terminal SH2 domain responsible for homo- or herterodimerization.
Distribution

Cytoplasm.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
Interaction
STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lufei C, et al. 2003. EMBO J. 22:1325.
2. Deo D, et al. 2002. J. Biol. Chem. 277:21237.
3. Pfeffer L, et al. 1997. Science 276:1418.
4. Akira S, et al. 1994. Cell 77:63.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org
Go To Top Version: 7    Revision Date: 04/23/2019

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