- Clone
- P83B5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
- Isotype
- Mouse IgG1, κ
-
Total cell lysates (15 µg protein) from Raw264.7 (lane 1), PC3 (lane 2) and HeLa (lane 3) and Molt-4 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of Purified anti-STAT3 Antibody (clone P83B5) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-STAT3 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1(lower panel) as a loading control. Lane M: Molecular weight ladder. -
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT3 (clone P83B5), or B) equal amount of purified Mouse IgG1, κ isotype control antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 691502 | 100 µg | $253.00 | |||
STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant fragment (aa 20-240) of human STAT3 expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ChIP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested use of this reagent is 1-50-1:150 dilution by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The canonical sequence of STAT3 contains 770 amino acids with a predicted molecular weight of 88 kD. A shorter isoform of STAT3 with 722 amino acids (83 kD) was reported. Clone P83B5 may recognize both isoforms.
- RRID
-
AB_2632650 (BioLegend Cat. No. 691502)
Antigen Details
- Structure
- 770 amino acids with a predicted molecular weight of 88.1 kD that contains a C-terminal SH2 domain responsible for homo- or herterodimerization.
- Distribution
-
Cytoplasm.
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
- Interaction
- STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lufei C, et al. 2003. EMBO J. 22:1325.
2. Deo D, et al. 2002. J. Biol. Chem. 277:21237.
3. Pfeffer L, et al. 1997. Science. 276:1418.
4. Akira S, et al. 1994. Cell. 77:63. - Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 on UniProt.org
Other Formats
View All STAT3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-STAT3 | P83B5 | WB,ChIP |
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