- Clone
- SYK-01 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Isotype
- Mouse IgG1, κ
-
Total cell lysates (15 µg total protein) from A549 (negative control), HeLa (negative control), HEL, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Syk antibody, clone SYK-01, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SYK-01 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 626201 | 25 µg | $118.00 | |||
| 626202 | 100 µg | $293.00 | |||
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Product Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant (amino acids 5-360) human Syk
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation.
This clone may produce a non-specific band of roughly ~250 kD at higher antibody concentrations
This clone does not recognize ZAP70
This clone has been reported to recognize rat Syk -
Application References
(PubMed link indicates BioLegend citation) -
- Arudchandran R, et al. 2000. J. Exp. Med. 191:47. (IP WB)
- Swann PG, et al. 1999. J. Biol. Chem. 33:23068. (IP)
- Inoue M, et al. 2011. J. Immunol. 186:18. PubMed
- Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed
- Cunningham CC, et al. 2012. Clin Immunol. 144:228. PubMed
- Alsadeg A, et al. 2014. J Immunol. 193:268. PubMed
- Product Citations
-
- RRID
-
AB_2200268 (BioLegend Cat. No. 626201)
AB_2200268 (BioLegend Cat. No. 626202)
Antigen Details
- Structure
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
- Distribution
-
Cytoplasmic protein, widely expressed in hematopoietic cells
- Function
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- Interaction
- c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, paxillin, FAK, Grb2, CD3ε, LAT, many others
- Modification
- Phosphorylation (Y131, Y323, Y348, Y352, Y525, Y526)
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe S-L, et al. 2001.Immunology 103:164. - Gene ID
- 6850 View all products for this Gene ID
- UniProt
- View information about Syk on UniProt.org
Other Formats
View All Syk Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Syk | SYK-01 | WB,IHC-P,IP |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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