Purified anti-Syk Antibody

Pricing & Availability
Clone
5F5 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype
Mouse IgG1, κ
5F5_Purified_Syk_Antibody_1_032019
Total cell lysates (15 µg total protein) from A549, RPMI-8226, Daudi , and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-Syk Antibodies clones SYK-01 (0.5 µg/mL) and clone 5F5 (1.0 µg/mL), overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 5F5_Purified_Syk_Antibody_1_032019
    Total cell lysates (15 µg total protein) from A549, RPMI-8226, Daudi , and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-Syk Antibodies clones SYK-01 (0.5 µg/mL) and clone 5F5 (1.0 µg/mL), overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-WB_5F5_061609
    Mouse spleen (1) and PBMC (2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with clone 5F5 (mouse-specific). Proteins were visualized using an HRP goat anti-mouse secondary antibody conjugate and a chemiluminescent system.
Cat # Size Price Quantity Check Availability
646001 25 µg $124.00
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646002 100 µg $265.00
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Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Syk peptide aa306-333
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Additional Product Notes

In the first WB figure above, the cell lines selected  for antibody testing were chosen to demonstrate specificity and species cross-reactivity, A549 was chosen as a human control cell line that does not express Syk protein; RPMI 8225 and Daudi are human cell lines that express Syk protein; and A20 cells is a mouse cell line that expresses Syk protein. Predicted SYK expression was obtained from Human Protein Atlas.

Application References

(PubMed link indicates BioLegend citation)
  1. Chu D, et al. 1999. J. Immunol. 163:2610.
  2. Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
Product Citations
  1. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  2. Ackermann J, et al. 2015. J Immunol. 194:4650. PubMed
  3. Németh T, et al. 2016. Nat Commun. 7: 11004. PubMed
RRID
AB_1659262 (BioLegend Cat. No. 646001)
AB_1659262 (BioLegend Cat. No. 646002)

Antigen Details

Structure
Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution

Cytoplasmic protein, widely expressed in hematopoietic cells

Function
Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.

Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk on UniProt.org
Go To Top Version: 2    Revision Date: 03/20/2019

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