- Clone
- 20A1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
- Isotype
- Mouse IgG1, κ
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Whole cell lysates (15 µg protein) from HeLa, HEK293, and RAW264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-TIF1β (KAP-1, TRIM28) antibody, clone 20A1. Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse IgG secondary antibody (1:3000 dilution, Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TRIM28 mRNA expression levels; HEK293 cells express ~8-fold more TRIM28 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker. -
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-TIF1β (KAP-1, TRIM28) (clone 20A1) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 619301 | 25 µg | $101.00 | |||
| 619302 | 100 µg | $218.00 | |||
TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
ChIP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg/mL. -
Application References
(PubMed link indicates BioLegend citation) -
- Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
- Product Citations
-
- RRID
-
AB_2209724 (BioLegend Cat. No. 619301)
AB_2209724 (BioLegend Cat. No. 619302)
Antigen Details
- Structure
- Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
- Distribution
-
Nuclear, associates with specific chromatin regions
- Function
- Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596. - Gene ID
- 10155 View all products for this Gene ID
- UniProt
- View information about TIF1beta on UniProt.org
Other Formats
View All TIF1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-TIF1β (KAP-1, TRIM28) | 20A1 | WB,ICC,ChIP |
| Alexa Fluor® 647 anti-TIF1β (KAP-1, TRIM28) | 20A1 | ICC |
| Alexa Fluor® 488 anti-TIF1β (KAP-1, TRIM28) | 20A1 | ICC |
| Brilliant Violet 421™ anti-TIF1β (KAP-1, TRIM28) | 20A1 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-TIF1β (KAP-1, TRIM28)
Whole cell lysates (15 µg protein) from HeLa, HEK293, and RA... 
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... -
Alexa Fluor® 647 anti-TIF1β (KAP-1, TRIM28)
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Alexa Fluor® 488 anti-TIF1β (KAP-1, TRIM28)
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Brilliant Violet 421™ anti-TIF1β (KAP-1, TRIM28)
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten...