- Clone
- W19027A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tripartite Motif Containing 28; RING Finger Protein 96; Transcription Intermediary Factor1-β, KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; KRAB-Associated Protein 1
- Isotype
- Rat IgG2a, κ
-
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-TIF1β (KAP-1, TRIM28) antibody (clone W19027A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 6 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg (1:100 dilution) purified rat IgG2a, κ isotype ctrl antibody (panel A) (Cat. No. 400501) or purified anti-TIF1β (KAP-1, TRIM28) antibody (clone W19027A) (panel B) overnight at 4°C followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. -
Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400501) or purified anti-TIF1β (KAP-1, TRIM28) antibody (clone W19027A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-TIF1β (KAP-1, TRIM28) antibody (clone 20A1) (Cat. No. 619301). Lane M: Molecular weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 941301 | 25 µg | $118.00 | |||
| 941302 | 100 µg | $293.00 | |||
TIFβ (transcription intermediary factor 1-beta) is an 89 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full-length recombinant mouse TIF1β protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
- BioLegend offers two monoclonal antibodies, clones 20A1 and W19027A that bind TIF1β (KAP-1, TRIM28).
- 20A1 is a mouse IgG1, κ isotype; W19027A is a rat IgG2a, κ isotype
- Both clones detect human and mouse TIF1β by western blot; 20A1 binds TIF1β with a higher affinity than W19027A for this application, but also shows a higher background
- Both clones are validated for immunocytochemistry on human cells
- W19027A is validated for immunoprecipitation, 20A1 has not been tested for this application
- Clone W19027A was tested for immunocytochemistry using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. Both methods were compatible with TIF1β staining. - RRID
-
AB_2888901 (BioLegend Cat. No. 941301)
AB_2888901 (BioLegend Cat. No. 941302)
Antigen Details
- Structure
- TIF1β is an 835 amino acid protein with a predicted molecular weight of 89 kD.
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Chromatin remodeling
- Antigen References
-
1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596. - Gene ID
- 10155 View all products for this Gene ID
- UniProt
- View information about TIF1beta on UniProt.org
Other Formats
View All TIF1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-TIF1β (KAP-1, TRIM28) Antibody | W19027A | WB,IP,ICC |
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