- Clone
- 11G10SC (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TRIM28 pS473, KAP-1 pS473
- Isotype
- Mouse IgG1, κ
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Total cell lysates (15 µg total protein) from HEK293T cells and untreated (-) or treated (+) with UV radiation were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody, clone 11G10SC, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-TIF1β (KAP-1, TRIM28) Antibody, clone 20A1 (Cat. 619302), was used as a pan TIF1β loading control at 1:50000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
HEK293 cell extracts untreated (lane 1) and treated with UV radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β phosphorylated (Ser473) antibody (clone 11G10SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 654101 | 25 µg | $118.00 | |||
| 654102 | 100 µg | $293.00 | |||
Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1β is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Modified peptide: VKRSRpSGEGEVC.
- Formulation
-
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2561781 (BioLegend Cat. No. 654101)
AB_2561781 (BioLegend Cat. No. 654102)
Antigen Details
- Structure
- An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus.
- Distribution
-
Nucleus
- Function
- TIF1β acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins.
- Interaction
- TIF1β was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1α or TIF1γ.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Messerschmidt DM, et al. 2012. Science 335:1499.
2. Chikuma S, et al. 2012. Nat. Immunol. 13:596. - Gene ID
- 21849 View all products for this Gene ID
- UniProt
- View information about TIF1beta on UniProt.org
Other Formats
View All TIF1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-TIF1β (KAP-1, TRIM28) Phospho (Ser473) | 11G10SC | WB,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-TIF1β (KAP-1, TRIM28) Phospho (Ser473)
HEK293 cell extracts untreated (lane 1) and treated with UV ... 
Total cell lysates (15 µg total protein) from HEK293T cells ...