- Clone
- Poly6209 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- γ-tubulin
- Isotype
- Rabbit Polyclonal IgG
-
Total cell lysates (15 µg total protein) from A549, U87-MG, NTERA-2, and U937 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Purified anti-Tubulin-γ Antibody, clone Poly6209, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
Whole cell extract from A431 cells (Lane 1) and NIH3T3 cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-γ-tubulin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 620901 | 50 µL | $89.00 | |||
| 620902 | 200 µL | $265.00 | |||
γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1. The Poly6209 antibody has been shown to be useful for Western blotting of human and mouse γ-tubulin.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Peptide-KLH
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- Preparation
- The antibody was purified by antigen-affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
- Application
-
WB - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 - 1:8000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes. - Additional Product Notes
-
The 50 µL size can be used for approximately 10 Western blots, and the 200 µL size can be used for approximately 40 Western blots.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
SCR_001134 (BioLegend Cat. No. 620901)
SCR_001134 (BioLegend Cat. No. 620902)
Antigen Details
- Structure
- Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
- Distribution
-
Cytoskeletal protein, high expressed in centrosomes
- Function
- Cytoskeletal organization, mitosis
- Interaction
- Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
- Modification
- Phosphorylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Zheng Y, et al. 1991. Cell 65:817.
- Regulation
- Expressed at highest levels during prophase to metaphase
- Gene ID
- 2276 View all products for this Gene ID
- UniProt
- View information about Tubulin-gamma on UniProt.org
Other Formats
View All Tubulin-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tubulin-γ | Poly6209 | WB,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
