- Clone
- Poly29191 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CTRCT30, HEL113, epididymis luminal protein 113
- Previously
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Covance Catalog# PCK-594P
- Isotype
- Chicken IgY
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Immunohistochemical staining of Vimentin , using Poly29191 on formalin fixed paraffin embedded human tonsil tissue. -
Total lysates (15 µg protein) from HeLa (Human), Raw264.7 (Mouse) and UMR106 (Rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:5000 purified Vimentin antibody, clone Poly29191. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-chicken secondary antibody (1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution). -
Total cell lysates (15 µg total protein) from Daudi (negative control), PC-3, Jurkat and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5,000 dilution) of Purified anti-Vimentin Antibody, clone Poly29191, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP conjugated donkey anti-Chicken IgY antibody. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
Immunofluorescence of A431 cells with (A) Chicken IgY isotype control (Negative, Cat. No. 402101) or (B-D) Vimentin primary antibody (clone Poly29191). Alexa Fluor® 488 (Green) Goat anti-Chicken IgY was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/15, and (B-D) is 1/70. Concentrations for (A, B) is 1/1000, (C) is 1/2500, (D) is 1/5000.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 919101 | 100 µL | $313.00 | |||
Vimentin is a protein subunit of the 10nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells, several types of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.
Product Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Chicken
- Immunogen
- This antibody was raised in chickens against purified recombinant vimentin.
- Formulation
- Phosphate-buffered solution, containing 10 mM sodium azide.
- Preparation
- The antibody is an IgY preparation.
- Concentration
- ~20 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:2000 - 1:8000 dilution. For immunocytochemistry, a dilution range of 1:1000 - 1:5000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunofluorescence (IF) and immunohistochemistry (IHC). This antibody does not bind to protein A or protein G.
Predicted MW is ~54 kD
-
Application References
(PubMed link indicates BioLegend citation) -
- Herrmann H, et. al. 1989. Development 105:279. (IF, ICC, IHC, WB)
- KivelS T, et. al. 1992. J. Histochem. Cytochem. 40:1517. (IHC)
- Stathopoulos, et. al. 1989. J. Histochem. Cytochem. 37:1363. (IHC)
- Product Citations
-
- RRID
-
AB_2565208 (BioLegend Cat. No. 919101)
Antigen Details
- Cell Type
- Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Intermediate Filaments
- Gene ID
- 7431 View all products for this Gene ID
- UniProt
- View information about Vimentin on UniProt.org
Other Formats
View All Vimentin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Vimentin | Poly29191 | IHC-P,WB,ICC |
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