- Clone
- 9D11A43 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- XBP-1 isoform2, Tax-responsive element-binding protein 5 (TREB5), XBP2
- Isotype
- Mouse IgG2a, κ
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Total cell lysates (15 µg protein) from HepG2 cells treated without (-) or with (+) 300 nM thapsigargan for the indicated timepoints were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Xbp-1s antibodies (clones 9D11A43 and 143F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular weight ladder. -
Western blot analysis of HepG2 untreated (lane 1) cell, HepG2 treated with 300 nM of thapsigargin for overnight (lane 2), Raw264.7 untreated (lane 3) and Raw264.7 treated with 300 nM of thapsigargin for overnight (lane 4) using anti-XBP-1s antibody (clone 9D11A43). Purified anti-GAPDH antibody (Poly6314) was used as a loading control. -
HepG2 cells treated with thapsigargin (300nM, 16 hours) were stained with purified anti-XBP-1s (9D11A43) antibody, followed by staining with DyLight 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue). -
Chromatin Immunoprecipitations (ChIP) were performed using fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2.0 µg/mL, 8 hr). ChIP was performed with 10.0 µg of chromatin and 2.0 µg of purified anti- XBP-1s antibody (clone 9D11A43) or Go-ChIP-Grade™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400183). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human DNAJB9 exon 1 region and the human α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 658802 | 100 µg | $265.00 | |||
XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of the human HLA-DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 is upregulated as part of the ER stress response, also known as the unfolded protein response.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial mouse XBP-1s recombinant protein (162-267aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ChIP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For ChIP application, use 2 µg of antibody and 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The clone 9D11A43 recognizes spliced XBP-1 (XBP-1s/isoform 2), which is 371 amino acids and migrates around 55 kD on the SDS-PAGE.
- Additional Product Notes
-
Using this antibody at a ratio higher than 1:5 (µg antibody per ug of chromatin) will increase background
- Product Citations
-
- RRID
-
AB_2562960 (BioLegend Cat. No. 658802)
Antigen Details
- Distribution
-
Nucleus.
- Function
- Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- MHC Antigens, Nuclear Markers
- Antigen References
-
1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154. - Gene ID
- 7494 View all products for this Gene ID
- UniProt
- View information about XBP-1s on UniProt.org
Other Formats
View All XBP-1s Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-XBP-1s | 9D11A43 | WB,ICC,ChIP |
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