C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD86 PE. Live cells were gated for analysis.
C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 2 minutes, fixed with Fixation buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, then stained with B220 Brilliant Violet 421™ and ZAP70 Phospho (Tyr319)/Syk (Tyr352) PE
Poly21571 reacts with mouse IgM. It can be used in functional assays, such as B cell activation. It has minimum cross-reactivity with bovine proteins. The optimal working concentration should be determined empirically based on the specific assay of interest.
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
Preparation
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. The solution contains no preservative; handle under aseptic conditions.
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