- Regulatory Status
- RUO
- Other Names
- CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
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Immobilized recombinant human B7-2/CD86 binds biotinylated human CTLA-4 in a dose dependent manner with EC50 of 10 - 40 ng/mL. -
Stability testing for Human B7-2/CD86. Human B7-2/CD86 was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to bind biotinylated human CTLA-4.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 772902 | 10 µg | $71.00 | |||
| 772904 | 25 µg | $106.00 | |||
| 772906 | 100 µg | $223.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD86, also known as B7-2, is a member of cell surface immunoglobulin superfamily. B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2. These members are either co-stimulatory or co-inhibitory in modulating T cell response. Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1. These members are involved in regulating induction and maintenance of peripheral immune tolerance. Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Product Details
- Source
- Human B7-2/CD86, amino acid Leu26-Pro247 (accession # NP_787058.4) with a C-terminal 6x His tag, was expressed in 293E cells.
- Molecular Mass
- The 228 amino acid recombinant protein has a predicted molecular mass of approximately 26.21 kD. The DTT-reduced and non-reduced protein migrates at approximately 52 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 1x PBS.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When human B7-2/CD86 is immobilized at 0.5 μg/ml (100 μl/well) using a anti-His antibody (Cat. No. 652501), biotinylated human CTLA-4 binds with EC50 of 10 - 40 ng/ml in a functional ELISA.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Immunoglobulin family
- Distribution
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Activated antigen presenting cells, including B cells, dendritic cells and macrophages
- Function
- B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
- Interaction
- Leukocytes, endothelial cells
- Ligand/Receptor
- CD28, CTLA4
- Bioactivity
- Measured by the ability of immobilized protein to bind biotinylated human CTLA-4
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Gene ID
- 942 View all products for this Gene ID
- UniProt
- View information about B7-2/CD86 on UniProt.org